摘要
以‘嘉粉200’为试验品种,探讨微咸水对番茄叶片与根系生理特性的影响。设置4种微咸水灌溉浓度:CK(NaCl 0 g/L)、T1(NaCl 1 g/L)、T2(NaCl 2 g/L)、T3(NaCl 3 g/L),对番茄的株高、茎粗,根系指标,荧光作用参数(光化学淬灭系数(qP)、PSⅡ原初光能转换效率(Fv/Fm)和非光化学淬灭系数(qN),光合参数蒸腾速率(T_(r))、净光合速率(P_(n))和胞间二氧化碳浓度(Ci)和不同位置叶片根系的抗氧化酶(超氧化物歧化酶(SOD)、过氧化物酶(POD)和过氧化氢酶(CAT)进行研究。结果表明:微咸水处理抑制番茄株高、叶绿素含量和根长;T_(r)降低,P_(n)和C_(i)先降后升,qP和Fv/Fm下降而qN上升;在结果期时,随着微咸水灌溉浓度的增加,SOD、POD和CAT活性含量呈现先增后减的趋势;根系SOD随着微咸水灌溉浓度的增加而增加,POD含量与CAT含量都随着微咸水灌溉浓度的增加呈现先增加后减少的趋势;根系SOD与幼苗期和开花期叶片的SOD均存在极显著差异(P<0.01),与3个时期叶片的CAT存在显著差异(P<0.05);根系POD与开花期叶片的SOD存在极显著差异(P<0.01),与幼苗期叶片的SOD和开花期叶片的CAT存在显著差异(P<0.05);根系CAT与幼苗期和开花期的SOD存在极显著差异(P<0.01)。微咸水处理抑制植株生长状态,番茄植株为抵御逆境胁迫,自身的抗氧化酶系统会被激活,根系与叶片的抗氧化酶存在相关性。
The study aimed to investigate the effects of brackish water on the physiological characteristics of tomato leaves and roots using the‘Jiafen 200’variety as the subject.Treatments were set as follows:CK(NaCl 0 g/L),T1(NaCl 1 g/L),T2(NaCl 2 g/L),and T3(NaCl 3 g/L).Different shoot and root parameters of tomato plants were examined,including plant growth indicators(plant height,shoot diameter,and root system assessment);fluorescence parameters(fluorescence parameters(photochemical quenching coefficient(qP),maximum photochemical yield(F_(v)/F_(m)),and nonphotochemical quenching coefficient(qN);photosynthetic parameters(transpiration rate(T_(r)),net photosynthetic rate(P_(n)),and intercellular carbon dioxide concentration(C_(i));and root antioxidant enzyme activity(superoxide dismutase(SOD),peroxidase(POD),and catalase(CAT).Treatment with brackish water inhibited plant height,chlorophyll content,and root length in tomatoes.The Tr value decreased;Pn and Ci initially decreased and then increased;qP and Fv/Fm values decreased;and qN increased under brackish water treatment.During the fruiting period,as the concentration of brackish water irrigation increased,the activities of SOD,POD,and CAT in the roots first increased and then decreased.The root SOD activity increased with higher concentrations of brackish water irrigation,while POD and CAT activities showed a trend of initially increasing and then decreasing.There were significant correlations between root and leaf SOD activities at the seedling and flowering stages(P<0.01).Significant correlations were also observed between root SOD activity and leaf CAT activity at the seedling,flowering,and fruiting stages(P<0.05).Root POD activity showed significant orrelations with SOD activity at the flowering stage(P<0.01),and with SOD activity at the seedling stage and CAT activity at the flowering stage(P<0.05).Root CAT activity exhibited highly significant correlations with SOD activity at the seedling and flowering stages(P<0.01).These results suggest that antioxidant enzyme activities in tomato plants are activated in response to salt stress,with a clear relationship between the antioxidant activities in roots and leaves.
作者
张祎洋
朱彦哲
马思艳
马燕
王静
吴龙国
ZHANG Yiyang;ZHU Yanzhe;MA Siyan;MA Yan;WANG Jing and WU Longguo(College of Wine and Horticulture,Ningxia University,Yinchuan 750021,China)
出处
《西北农业学报》
北大核心
2025年第8期1446-1454,共9页
Acta Agriculturae Boreali-occidentalis Sinica
基金
宁夏高等学校自然科学项目(NYG-2024-019)
宁夏青年拔尖人才项目
宁夏自然科学基金(2024AAC03120)
国家重点研发计划项目子课题(2021YFD1600302-3)。
关键词
番茄
微咸水
抗氧化酶
光合参数
钠盐胁迫
Tomato
Brackish water
Antioxidant enzymes
Photosynthetic parameters
Sodium salt stress
作者简介
第一作者:张祎洋,女,硕士研究生,从事番茄逆境胁迫的研究。E-mail:zyy13781010198@163.com;通信作者:吴龙国,男,讲师,从事园艺作物表型信息感知与智能装备研制的研究。E-mail:wlg@nxu.edu.cn。
