摘要
为探究太阳长棘海星(Acanthaster cf.solaris)对鹿角杯形珊瑚(Pocillopora damicornis)的摄食作用及其分子调控机制,本研究以南海典型的鹿角杯形珊瑚为研究对象,结合行为学实验、转录组测序及胃组织酶活性测定,系统分析了嗅觉受体在其摄食行为中的作用。结果表明,太阳长棘海星在摄食鹿角杯形珊瑚后,化学感受器的基因表达发生显著变化,其中89个基因的表达发生显著上调,335个基因发生显著下调。深入挖掘表达上调基因,发现gamma-aminobutyric acid type B receptor subunit 1-like,olfactory receptor 13-like等5个转录组提示的嗅觉受体基因可能在海星识别和摄食鹿角杯形珊瑚过程中发挥了关键作用。同时,摄食行为显著激活胃组织中纤维素酶活性(p<0.05),而磷酸烯醇式丙酮酸羧化酶和脂肪酶活性也呈增大趋势但未达显著水平,表明纤维素酶可能通过增强对珊瑚和虫黄藻细胞壁的分解能力参与消化过程。本研究初步揭示了太阳长棘海星关键嗅觉受体对摄食行为的介导机制,为深入解析其种群暴发机理提供了重要科学参考。
To investigate the feeding effects of Acanthaster cf.solaris on Pocillopora damicornis and its molecular regulatory mechanisms,this study focused on the typical P.damicornis in the South China Sea,combining behavioral experiments,transcriptome sequencing,and enzyme activity assays of stomach tissue to systematically analyze the role of olfactory receptors in the feeding behavior of A.cf.solaris.The results showed that the expression of chemical receptor genes in the A.cf.solaris significantly changed during the feeding process on P.damicornis,with 89 genes being significantly upregulated and 335 genes being significantly downregulated.Further exploration of the upregulated genes revealed that five olfactory receptor genes suggested by the transcriptome,including gamma-aminobutyric acid type B receptor subunit 1-like and olfactory receptor 13-like,may play crucial roles in the recognition and feeding on P.damicornis.Meanwhile,feeding behavior significantly activated cellulase activity in the stomach tissues(p<0.05),whereas the activities of phosphoenolpyruvate carboxykinase and lipase showed an increasing trend but did not reach statistical significance.This suggests that cellulase may be involved in the digestive process by enhancing the degradation of coral skeletons and zooxanthella cell walls.This study provides preliminary insights into the mediation of feeding behavior by key olfactory receptors in Acanthaster cf.solaris and offers important scientific references for further understanding the mechanisms behind population outbreaks.
作者
朱云杰
张志伟
邢家杰
闫智聪
蔡文启
吴钟解
刘兆群
周智
Zhu Yunjie;Zhang Zhiwei;Xing Jiajie;Yan Zhicong;Cai Wenqi;Wu Zhongjie;Liu Zhaoqun;Zhou Zhi(School of Ecology,Hainan University,Haikou 570228,China;School of Marine Science and Engineering,Hainan University,Haikou 570228,China;School of Marine Biology and Fisheries,Hainan University,Haikou 570228,China;Hainan Academy of Ocean and Fisheries Sciences,Haikou 571126,China)
出处
《海洋学报》
北大核心
2025年第5期40-47,共8页
基金
国家重点研发计划(2022YFC_(31)06302)。
关键词
长棘海星
嗅觉受体
造礁珊瑚
摄食行为
转录组
Crown-of-thorns starfish
olfactory receptors
reef-building corals
feeding mechanism
transcriptome
作者简介
朱云杰(1994-),男,河南省郑州市人,主要从事太阳长棘海星群体聚集机制研究。E-mail:zyj20240@163.com;通信作者:刘兆群,教授,博士生导师,主要从事珊瑚礁资源利用研究。E-mail:liuzhaoqun@hainanu.edu.cn;通信作者:周智,研究员,博士生导师,主要从事珊瑚礁保护研究。E-mail:zhouzhi@hainanu.edu.cn。
