摘要
目的比较两种常用Rho激酶抑制剂Fasudil与Y27632促进皮神经再生的效能。方法SD大鼠15只,建立隐神经钳夹模型并分入对照组、Fasudil组与Y27632组,分别给予DMSO、Fasudil(40 mg/kg)及Y27632(40 mg/kg)腹腔注射,1次/d。术后7 d,隐神经新生轴突染色。15只ICR小鼠,切取背部穿支皮瓣,将小鼠等分入如上3组并相应给药。30 d后,对皮瓣内轴突进行定量分析。提取大鼠胚胎背根神经节(DRG),置PDMS模具中培养,构建体外轴突切断模型,后分入以上3组,并相应给药。3 d后测量DRG轴突再生长度。单因素方差分析统计处理。结果隐神经损伤1周后,对照、Fasudil及Y27632组新生轴突密度分别为(43.2±6.5)%、(55.8±5.2)%及(68.0±2.3)%。Y27632组轴突密度显著高于其它2组(P<0.01)。皮瓣术后1个月,对照、Fasudil及Y27632组皮瓣远端横截面的神经密度分别为(1.1±1.1)‰,(2.8±0.5)‰及(6.0±0.9)‰,Y27632组皮瓣的神经密度显著高于另外2组(P<0.001)。DRG轴突切断3 d后,SPRR1a、ATF3、c-JUN、GAP43、RhoA和ROCK2的表达量显著增加。此外,对照、Fasudil及Y27632组DRG的再生轴突长度与生长锥面积分别为(1916±132)μm和(40±7)μm^(2)、(2302±134)μm和(55±7)μm^(2)及(2662±112)μm和(79±5)μm^(2)。Y27632组DRG再生轴突长度和生长锥面积显著大于其它2组(P<0.05)。结论Y27632具有比Fasudil更强促进DRG生长锥扩展及皮神经损伤后修复的能力,非常适合用于促进皮瓣术感觉恢复。
Objective To compare effects of two commonly used Rho kinase inhibitors,Fasudil and Y27632,on promoting cutaneous nerve regeneration.Methods Firstly,15 Sprague Dawley rats were used to establish a clamp-induced injury model on the saphenous nerve.They were then divided into the control,Fasudil and Y27632 groups,and intraperitoneal given DMSO,Fasudil(40 mg/kg)or Y27632(40 mg/kg),respectively,once a day for 7 days after surgery.Then,the regenerated axons within the saphenous nerve were evaluated.Secondly,perforator flaps were harvested on the back of 15 ICR mice,which were equally divided into the above-mentioned three groups with drugs administered accordingly.30 days later,axons within the flaps were quantitatively analyzed.Finally,the rat embryonic dorsal root ganglias(DRG)were collected and cultured in PDMS molds to establish an in vitro axotomy model,which were then divided into the abovementioned three groups and treated accordingly.The length of regenerated DRG axons was measured after 3 days.One-way analysis of variance was used to process the data.Results One week after saphenous nerve injury,the density of new axons in the control,Fasudil and Y27632 groups was(43.2±6.5)%,(55.8±5.2)%and(68.0±2.3)%,respectively.The axon density of the Y27632 group was significantly higher than those of the other 2 groups(P<0.01).One month after flap surgery,the nerve density of the distal cross-section of the flap in the control,Fasudil and Y27632 was(1.1±1.1)‰,(2.8±0.5)‰and(6.0±0.9)‰,respectively.The nerve density of the flap in the Y27632 group was significantly higher than the other 2 groups(P<0.001).3 days after DRG axotomy,the expression of SPRR1a,ATF3,c-JUN,GAP43,RhoA and ROCK2 significantly increased.The length of the regenerated axons and the area of growth cones of DRG in the control,Fasudil and Y27632 groups were(1916±132)μm and(40±7)μm^(2),(2302±134)μm and(55±7)μm^(2),and(2662±112)μm and(79±5)μm^(2),respectively.The length of the regenerated axons and the area of growth cones of DRG in the Y27632 group were significantly larger than that of the other 2 groups(P<0.05).Conclusions Y27632 is more capable than Fasudil to promote DRG growth cone expansion and repair after cutaneous nerve injury,and is very suitable for promoting sensory recovery after skin flap surgery.
作者
王燕亭
占少琴
方芳
庄跃宏
刘东红
Wang Yanting;Zhan Shaoqin;Fang Fang;Zhuang Yuehong;Liu Donghong(Education People's Livelihood College,Xiamen City University,Xiamen 361001,Fujian Province,China;Engineering Research Center of Natural Cosmeceuticals College of Fujian Province,Xiamen Medical College,Xiamen 361023,Fujian Province,China;Fujian Provincial Key Laboratory of Brain Aging and Neurodegenerative Diseases,Institute of Clinical Applied Anatomy,Fujian Medical University,Fuzhou 350122,Fujian Province,China;Department of Burn,Plastic and Aesthetic Surgery,The Second Affiliated Hospital of Shandong First Medical University,Tai'an 271000,Shandong Province,China)
出处
《中国临床解剖学杂志》
北大核心
2025年第3期289-296,共8页
Chinese Journal of Clinical Anatomy
基金
厦门医学院科技计划项目(K2023-30)
天然化妆品福建省高校工程研究中心(厦门医学院)开放课题(XMMC-NC202104)。
作者简介
王燕亭(1984-),女,福建厦门人,硕士研究生,主要从事皮瓣神经再支配的研究,E-mail:278296548@qq.com;通讯作者:刘东红,硕士,副主任医师,E-mail:39145958@qq.com。
