摘要
为提高常见食源性致病菌单核细胞增生李斯特氏菌、大肠埃希氏菌O157:H7、沙门氏菌的检测效率,本研究与前期研制的LES共增菌培养基联用,参考《出口食品中食源性致病菌检测方法实时荧光PCR法》(SN/T 1870—2016)方法,通过优化反应体系及反应条件,建立一种多重荧光PCR检测3种目标菌的方法。通过验证不同混合比例共增菌培养物检测效果及体系的特异性、敏感性,成功构建3种目标菌“同平台共增菌-多重荧光PCR检测”方法,体系的特异性好,灵敏度高,能够为食源性致病菌的快速检测提供技术支持。
In order to improve the detection efficiency of common foodborne pathogenic bacteria Listeria monocytogenes,Escherichia coli O157:H7 and Salmonella,this study was combined with the previously developed LES co-growth culture medium,referring to the SN/T 187—2016.By optimizing the reaction system and reaction conditions,a multiplex PCR method was established for the detection of three target bacteria.By verifying the detection effect of different mixed proportions of co-proliferating cultures and the specificity and sensitivity of the system,the“Co-proliferating with platform-multiple PCR detection”method for three target bacteria was successfully constructed.The system has good specificity and high sensitivity,and can provide technical support for the rapid detection of foodborne pathogens.
作者
劳嘉倩
蒋佳希
黄志深
尹玮璐
梁美丹
林秀敏
陈楷
LAO Jiaqian;JIANG Jiaxi;HUANG Zhishen;YIN Weilu;LIANG Meidan;LIN Xiumin;CHEN Kai(Guangzhou Institute for Food Inspection,Guangzhou 511400,China)
出处
《现代食品》
2025年第5期216-222,共7页
Modern Food
基金
广州市市场监督管理局科技项目(2023KJ36)。
作者简介
通信作者:劳嘉倩(1990-),女,本科,工程师,研究方向为食品微生物检验技术。E-mail:610137276@qq.com。
