摘要
目的:探讨云南某医院重症监护室(ICU)患者病原菌分布情况及屎肠球菌、金黄色葡萄球菌、肺炎克雷伯菌、鲍曼不动杆菌、铜绿假单胞菌和肠杆菌属细菌(以下简称“ESKAPE”)及大肠埃希菌的耐药性。方法:采集2020年6月至2021年6月云南某医院ICU患者临床标本,并对其进行分离培养,培养物经全自动微生物分析系统进行鉴定和药物敏感试验,采用WHONET 5.6、SPSS25.0软件对数据进行统计分析。结果:313例ICU患者的590份临床标本中共分离出病原菌675株,其中,革兰氏阴性菌480株(占71.1%),以肺炎克雷伯菌、鲍曼不动杆菌、大肠埃希菌为主;革兰氏阳性菌140株(占20.7%),以金黄色葡萄球菌为主;真菌55株(占8.2%),以白假丝酵母菌为主;共检出ESKAPE病原菌389株(占57.6%)。结论:该医院分离自ICU患者的前五位病原菌分别是肺炎克雷伯菌、鲍曼不动杆菌、大肠埃希菌、金黄色葡萄球菌和铜绿假单胞菌,均存在不同程度的耐药现象。
Objective:To explore the distribution of pathogenic bacterial and assess the antimicrobial resistance of ESKAPE pathogens(Enterococcus faecium,Staphylococcus aureus,Klebsiella pneumoniae,Acinetobacter baumanii,Pseudomonas aeruginosa,and Enterobacter spp.)and Escherichia coli in the intensive care unit(ICU)of a Yunnan hospital.Methods:Clinical specimens from ICU patients in a hospital in Yunnan from June 2020 to June 2021 were collected and isolated for culture.The cultures were identified and drug susceptibility tested by a fully automated microbial analysis system.WHONET 5.6 and SPSS 25.0 software were used for statistical analysis of data.Results:Among 590 specimens from 313 ICU patients,675 pathogenic strains were isolated.Gram-negative bacteria constituted 480 strains(71.1%),with Klebsiella pneumoniae,Acinetobacter baumannii,and Escherichia coli as predominant species.Gram-positive bacteria accounted for 140 strains(20.7%),mainly Staphylococcus aureus.Fungi comprised 55 strains(8.2%),primarily Candida albicans.ESKAPE pathogens constituted 57.6%of isolates.Conclusion:The top five pathogenic microorganisms in this ICU were Klebsiella pneumoniae,Acinetobacter baumannii,Escherichia coli,Staphylococcus aureus,and Pseudomonas aeruginosa,all exhibiting varying degrees of antimicrobial resistance.
作者
沈柏彤
魏奕菲
杨启明
王雨新
张宇恒
马立凡
武有聪
Shen Botong;Wei Yifei;Yang Qiming;Wang Yuxin;Zhang Yuheng;Ma Lifan;Wu Youcong(Pre-clinical College,Dali University,Dali,Yunnan 671000,China;Clinical College,Dali University,Dali,Yunnan 671000,China)
出处
《大理大学学报》
2024年第4期52-58,共7页
Journal of Dali University
基金
云南省大学生创新创业训练项目(202010679015)
云南省中青年学术和技术带头人后备人才项目(202305AC160038)。
关键词
重症监护室
病原菌分布
耐药性
云南
intensive care unit
pathogenic bacterial distribution
antimicrobial resistance
Yunnan
作者简介
第一作者:沈柏彤,硕士研究生,主要从事细菌感染与免疫研究;通信作者:武有聪,教授,博士,E-mail:youcongwu@163.com。
