摘要
目的:探究副干酪乳杆菌FZU103(Lactobacillus paracasei FZU103,LP-FZU103)对酒精性肝损伤(alcoholic liver injury,ALI)的防控作用。方法:选取36只SPF级ICR小鼠,随机分为空白组、模型组、实验组(LP-FZU103干预),实验6周后测定体质量及脏器系数、血清及肝脏生化、肝组织病理学及炎症因子、肝功能相关基因转录和肠道菌群组成。结果:与模型组相比,LP-FZU103干预可改善ALI小鼠的脏器系数和肝组织病理损伤程度,显著降低血清中总胆固醇、甘油三酯和低密度脂蛋白胆固醇浓度以及谷丙转氨酶和谷草转氨酶活性,提高血清高密度脂蛋白胆固醇浓度;显著提高肝脏中过氧化氢酶和超氧化物歧化酶活性以及谷胱甘肽含量,降低肝脏中丙二醛含量和白细胞介素6、干扰素γ水平;显著上调脂质代谢相关基因Ldlr及下调Acc1、Hmgcr和Cd36的mRNA水平;显著增加小鼠肠道中约氏乳杆菌(Lactobacillus johnsonii)、罗伊氏乳杆菌(Lactobacillus reuteri)、副干酪乳杆菌(Lactobacillus paracasei)等有益细菌的相对丰度。结论:LP-FZU103干预可一定程度防控小鼠ALI的发生,这与其改善肠道菌群及肝脏代谢功能密切相关。
Objective:To explore the preventive effect of Lactobacillus paracasei FZU103(LP-FZU103)on alcoholic liver injury(ALI).Methods:Altogether 36 specific pathogen free-(SPF-)grade ICR mice were randomly divided into three groups:control,model and experimental(LP-FZU103 intervention).After the six-week experiment,body mass,organ coefficients,serum and liver biochemical indexes,liver histopathology and inflammatory cytokines,the transcription of liver function-related genes and intestinal flora composition were measured.Results:Compared with the model group,intervention of LP-FZU103 improved the organ coefficients and pathological liver damage in ALI mice,significantly reduced the levels of total cholesterol(TC),triglyceride(TG)and low-density lipoprotein cholesterol(LDL-C)as well as the activity of alanine aminotransferase(ALT)and aspartate aminotransferase(AST)in the serum,increased the serum concentration of high-density lipoprotein cholesterol(HDL-C),significantly increased the activity of catalase(CAT)and superoxide dismutase(SOD)and the content of glutathione(GSH)in the liver,and decreased MDA content and interleukin-6(IL-6),interferon-γ(IFN-γ)levels in the liver.Moreover,LP-FZU103 intervention significantly up-regulated the mRNA expression level of the lipid metabolism-related gene Ldlr and down-regulated the mRNA expression level of Acc1,Hmgcr and Cd36 as well as increased the relative abundance of beneficial bacteria such as Lactobacillus johnsonii,Lactobacillus reuteri and Lactobacillus paracasei in the gut of mice.Conclusion:LP-FZU103 intervention can prevent and control the occurrence of alcoholic liver injury in mice,which is closely related to the improved intestinal flora and liver metabolic function.
作者
梁梓华
李嘉仪
谢林惠
杨梓翊
尤适泽
吴超
李文龙
艾连中
倪莉
吕旭聪
陈有挺
LIANG Zihua;LI Jiayi;XIE Linhui;YANG Ziyi;YOU Shize;WU Chao;LI Wenlong;AI Lianzhong;NI Li;LÜXucong;CHEN Youting(Institute of Food Science and Technology,College of Biological Science and Technology,Fuzhou University,Fuzhou 350108,China;Food Nutrition and Health Research Center,School of Advanced Manufacturing,Fuzhou University,Jinjiang 362200,China;The First Clinical Medical College,Fujian Medical University,Fuzhou 350004,China;Shanghai Engineering Research Center of Food Microbiology,School of Health Science and Engineering,University of Shanghai for Science and Technology,Shanghai 200093,China;Department of Hepatobiliary and Pancreatic Surgery,First Affiliated Hospital,Fujian Medical University,Fuzhou 350004,China)
出处
《食品科学》
EI
CAS
CSCD
北大核心
2024年第7期135-144,共10页
Food Science
基金
福建省教育与科研财政专项(BPB-2022CYT)
福州大学“旗山学者”高层次人才计划项目(GXRC21049)。
关键词
副干酪乳杆菌
酒精性肝损伤
氧化应激
肝功能基因
肠道菌群
Lactobacillus paracasei
alcoholic liver injury
oxidative stress
liver function-related genes
intestinal flora
作者简介
第一作者:梁梓华(2000—)(ORCID:0009-0002-5787-8490),男,硕士研究生,研究方向为食品生物技术。E-mail:1829381343@qq.com;通信作者:吕旭聪(1984—)(ORCID:0000-0002-0594-4284),男,研究员,博士,研究方向为食品发酵与酿造、健康食品生物制造。E-mail:xucong1154@163.com;通信作者:陈有挺(1973—)(ORCID:0000-0003-1446-5477),男,副教授,博士,研究方向为肝胆胰疾病的临床与基础。E-mail:chenyt1381@163.com。
