摘要
目的利用逆行跨单突触狂犬病毒(RV)系统,研究投射到脊髓背角(SDH)的蓝斑(LC)内去甲肾上腺素能神经元的突触前神经元在全脑的分布情况。方法第一步将二联辅助病毒注入到TH-Cre小鼠的LC中,两周后将重组RV注入到SDH中。小鼠存活一周后将其灌注取材,然后在激光共聚焦显微镜下观察LC-SDH通路的上游核团在全脑的分布情况。结果利用逆行跨单突触RV系统,可以在脑内的次级运动皮层(M2)、杏仁中央核(CeA)、中脑导水管周围灰质外侧区(lPAG)、中脑导水管腹外侧区(vlPAG)、中缝背核(DR)和巨细胞网状核(Gi)等核团内观察到RV阳性神经元,说明这些核团与LC-SDH通路构成了三级通路。结论脑内M2、CeA、lPAG、vlPAG、DR和Gi等核团与LC-SDH通路形成了三级通路。
Objective To explore the distribution of presynaptic neurons of noradrenergic neurons in the locus coeruleus(LC)projecting to the spinal dorsal horn(SDH)in the whole brain by using the retrograde transsynaptic rabies virus(RV)system.Methods In the first step,the double helper virus was injected into the LC of TH-Cre mice.Two weeks later,the recombinant RV was injected into SDH.After one week of survival,the mice were sacrificed,and then the distribution of upstream nuclei of LC-SDH pathway in the whole brain was observed under laser confocal microscopy.Results By using the retrograde transsynaptic RV system,RV-positive neurons could be observed in the secondary motor cortex(M2),central nucleus of the amygdala(CeA),lateral periaqueductal gray(lPAG),ventrolateral periaqueductal gray(vlPAG),dorsal raphe(DR)and gigantocellular reticular nucleus(Gi),indicating that these nuclei form a tertiary pathway with LC-SDH pathway.Conclusion The M2,CeA,lPAG,vlPAG,DR and Gi in the brain form a tertiary pathway with LC-SDH pathway.
作者
王欣瑶
薛茹
彭莉
张浚睿
李云鹏
刘蕊
张谅
WANG Xinyao;XUE Ru;PENG Li;ZHANG Junrui;LI Yunpeng;LIU Rui;ZHANG Liang(State Key Laboratory of Oral&Maxillofacial Reconstruction and Regeneration,National Clinical Research Center for Oral Diseases,Shaanxi Clinical Research Center for Oral Diseases,Department of Oral and Maxillofacial Surgery,School of Stomatology,Air Force Medical University,Xi'an 710032,China;Department of Nursing,School of Stomatology,Air Force Medical University,Xi'an 710032,China;Department of Anesthesiology,Tangdu Hospital,Air Force Medical University,Xi'an 710038,China)
出处
《空军军医大学学报》
CAS
2023年第11期1047-1051,共5页
Journal of Air Force Medical University
基金
陕西省重点研发计划国际科技合作计划项目重点项目(S2023-YF-GHZD-0108)
空军军医大学博士后启动基金(qd20220108)。
作者简介
王欣瑶,本科,主管护师,从事颌面外科护理研究,Tel:18066633510,E-mail:714013748@qq.com;通信作者:张谅,Tel:18189108609,E-mail:zlanesthesiologist@qq.com。
