摘要
为了获得甘薯抗盐突变体材料,本研究以平阳霉素(PYM)为诱变剂处理徐薯18的茎段获得甘薯突变体植株,并在NaCI培养基中筛选抗盐突变株系。结果表明,1.00 mg·L^(-1)PYM为徐薯18茎段诱变处理14 d后的半致死浓度。将徐薯18茎段接种至含不同浓度NaCl的MS培养基,结果表明125 mmol·L^(-1)NaCl为抗盐突变体的筛选浓度。最后,将经1.00 mg·L^(-1)PYM处理14 d后的甘薯植株的茎段接种到含125 mmol·L^(-1)NaCl的MS培养基,通过生根率筛选抗盐植株。盐胁迫下生根率最高的3株诱变植株的根长、株高、根鲜重、地上部鲜重、总鲜重、超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)活性及丙二醛(MDA)含量等指标的测定结果表明,这3株诱变植株的各形态和生理指标均显著优于诱变亲本徐薯18。隶属函数分析结果表明,这3株诱变植株的抗盐性均显著高于诱变亲本。与诱变亲本徐薯18相比,盐胁迫下3株突变体中的抗盐相关基因IbSOD、IbPOD、IbAPX和IbSOS的表达量均显著高于诱变亲本。本研究筛选到的抗盐甘薯突变体材料对甘薯抗盐新品种的培育及抗盐性遗传机理的研究具有重要意义。
In this study,Pinyamycin(PYM)was employed to generate sweetpotato mutants and salt-resistant mutants were screened.We found that the half lethality concentration of PYM was 1.00 mg·L^(-1)for Xushu 18 stem segments growing under MS medium for 14 days.And 125 mmol·L^(-1)NaCl was optimal for salt-resistant mutants screening.The sweetpotato materials treated with 1.00 mg·L^(-1)PYM for 14 days were inoculated into the screening medium containing 125 mmol·L^(-1)NaCl,and salt-resistant plants were screened by rooting rate.Three sweetpotato mutants with the highest rooting rate were selected and evaluated under NaCl stress.Based on the root length,plant height,fresh weight of root,fresh weight of above-ground,total fresh weight as well as the activities of superoxide dismutase(SOD),peroxidase(POD),catalase(CAT)and the content of malondialdehyde(MDA),the three mutants showed better morphological and physiological indexes than the parent Xushu 18.The membership function analysis showed that the three mutants had higher salt-resistance than the parent Xushu 18.Compared with the parent Xushu 18,the expression of IbSOD,IbPOD,IbAPX,and IbSOS genes related to salt-resistance was all significantly higher in the three mutants under salt stress.The salt-resistant sweetpotato mutants screened in this study are of great significance for the development of new sweetpotato varieties and the study on genetic mechanism of salt-resistance.
作者
韩婷婷
吕运韬
石源睿
刘世芳
唐锐敏
张红梅
贾小云
HAN Tingting;LYU Yuntao;SHI Yuanrui;LIU Shifang;TANG Ruimin;ZHANG Hongmei;JIA Xiaoyun(College of Life Sciences,Shanxi Agricultural University,Taigu,Shanxi 030801)
出处
《核农学报》
CAS
CSCD
北大核心
2023年第6期1111-1117,共7页
Journal of Nuclear Agricultural Sciences
基金
山西省农业科学院应用基础研究计划(YGC2019FZ4)
中央引导地方科技发展资金项目(2DYX20191400004408)
吕梁市引进高层次科技人才重点研发项目(2021RC-2-21)
2022年度高端外国专家引进计划(G2022004007L)。
关键词
甘薯
平阳霉素
诱变
抗盐突变体
sweetpotato
PYM
mutagenesis
salt-resistant mutants
作者简介
韩婷婷,女,主要从事植物重要功能基因的挖掘与应用。E-mail:hantingtin9@163.com;通讯作者:贾小云,女,教授,主要从事非编码RNA介导植物次生代谢物生物合成和逆境胁迫应答机制研究。E-mail:jiaxiaoyun@sxau.edu.cn。
