摘要
目的 探讨氧化苦参碱对溃疡性结肠炎(UC)模型小鼠体内炎性细胞因子表达与肠道菌群的影响。方法 2022年1—3月建立葡聚糖硫酸钠(DSS)诱导的UC模型。60只C57BL/6小鼠按照随机数字表法分为6组:空白组、模型组、5-氨基水杨酸组(50 mg/kg)、氧化苦参碱低剂量组(50 mg/kg)、氧化苦参碱中剂量组(100 mg/kg)、氧化苦参碱高剂量组(200 mg/kg),每组10只。根据小鼠体质量、粪便性状及便血情况,计算小鼠疾病活动指数(DAI)。酶联免疫法(ELISA)测定结肠组织中白细胞介素(IL)-6、IL-1β、肿瘤坏死因子α(TNF-α)表达,并采用16S rRNA评估小鼠肠道菌群结构的变化。结果 氧化苦参碱可降低小鼠DAI和结肠组织病理学评分。空白组、模型组、5-氨基水杨酸组、氧化苦参碱低、中、高剂量组IL-6含量分别为(29.64±5.31)ng/g、(111.31±11.47)ng/g、(44.97±7.22)ng/g、(100.67±11.44)ng/g、(74.12±6.55)ng/g、(68.78±6.98)ng/g;空白组、模型组、5-氨基水杨酸组、氧化苦参碱低、中、高剂量组IL-1β含量分别为(34.58±3.45)ng/g、(198.14±18.80)ng/g、(60.25±6.13)ng/g、(162.72±15.20) ng/g、(120.79±15.25)ng/g、(88.42±10.01)ng/g;空白组、模型组、5-氨基水杨酸组、氧化苦参碱低、中、高剂量组TNF-α含量分别为(50.57±4.50)ng/g、(298.68±11.47)ng/g、(44.97±7.22)ng/g、(100.67±11.44)ng/g、(74.12±6.55)ng/g、(68.78±6.98)ng/g。DSS使小鼠肠道菌群发生紊乱,多样性降低。经氧化苦参碱干预后,肠道菌群多样性明显增加;在门水平上,Firmicutes/Bacteroidetes、Actinobacteriota丰度显著提高,Proteobacteria丰度降低;在属水平上,Lactobacillus、Bifidobacterium和Akkermansia丰度显著升高,Escherichia-Shigella、Desulfovibrio、Blautia、Parasutterella、Turicibacter和Bacteroides丰度显著降低。结论 氧化苦参碱能够缓解UC症状,抑制UC小鼠炎性因子表达并调整其肠道菌群结构。
Objective To study the effect of oxymatrine on the expression of inflammatory cytokine and intestinal flora in mice with ulcerative colitis(UC).Methods A rat model of Dextran Sulfate Sodium Salt(DSS)-induced UC was established from January 2022 to March 2022.Sixty C57BL/6 mice were divided into 6 groups according to the random number method:blank group,model group,5-aminosalicylic acid group(50 mg/kg),low dose group(50 mg/kg),medium dose group(100 mg/kg),and high dose group(200 mg/kg),10 mg/kg per group.The DAI value was calculated based on body weight,fecal characteristics and hematochezia of mice.The protein expressions of IL-6,IL-1βand TNF-αwere determined.16S rDNA was carried out to estimate the gut microbiota.Results Oxymatrine could reduce DAI value and colonic histopathological score.The protein contents of IL-6 in the blank,model,5-aminosalicylic acid,oxymatrine low-dose,medium-dose and high-dose groups were(29.64±5.31)ng/g,(111.31±11.47)ng/g,(44.97±7.22)ng/g,(100.67±11.44)ng/g,(74.12±6.55)ng/g,and(68.78±6.98)ng/g,respectively.The protein contents of IL-1βin the blank,model,5-aminosalicylic acid,oxymatrine low-dose,medium-dose and high-dose groups were(34.58±3.45)ng/g,(198.14±18.80)ng/g,(60.25±6.13)ng/g,(162.72±15.20)ng/g,(120.79±15.25)ng/g,and(88.42±10.01)ng/g,respectively.The protein contents of TNF-αin the blank,model,5-aminosalicylic acid,oxymatrine low-dose,medium-dose and high-dose groups were(50.57±4.50)ng/g,(298.68±11.47)ng/g,(44.97±7.22)ng/g,(100.67±11.44)ng/g,(74.12±6.55)ng/g,and(68.78±6.98)ng/g,respectively.The results of 16S rRNA indicated that DSS caused disturbance of intestinal microflora and decreased its diversity.However,the diversity of gut microbiota was remarkably increased after oxymatrine treatment.At the phylum level,the abundance of Actinobacteriota and the ratio of Firmicutes to Bacteroidetes were significantly increased,and the abundance of Proteobacteria was obviously decreased.At the genus level,the abundances of Lactobacillus,Bi⁃fidobacterium and Akkermansia were remarkably increased,and the abundances of Escherichia-Shigella,Desulfovibrio,Blautia,Para⁃sutterella,Turicibacter and Bacteroides were significantly reduced.Conclusion Oxymatrine could alleviate the symptoms of UC mice,inhibit the expression of inflammatory factors and regulate the structure of gut microbiota.
作者
徐德荣
徐志红
陈磊垚
许立
XU Derong;XU Zhihong;CHEN Leiyao;XU Li(Department of Pharmacy,Nanjing Hospital of Integrated Traditional Chinese and Western Medicine(Affiliated Hospital of Nanjing University of Traditional Chinese Medicine),Nanjing,Jiangsu 210014,China;Jiangsu Key Laboratory for Pharmacology and Safety Evaluation of Chinese Materia Medica,Nanjing University of Chinese Medicine,Nanjing,Jiangsu 210046,China)
出处
《安徽医药》
CAS
2023年第6期1107-1111,I0004,共6页
Anhui Medical and Pharmaceutical Journal
作者简介
通信作者:徐志红,女,副主任中药师,研究方向为中药制剂及临床药学,Email:3578959690@qq.com。
