摘要
为更好地保存、研究和利用甘薯种质资源,本研究以国家甘薯种质资源圃(广州)保存的1091份甘薯种质为材料,分别采用欧氏距离和Nei’s距离进行NJ聚类分组,组内随机取样,构建核心种质。利用均值、方差、香农多样性指数、变异系数等指标对核心种质的表型性状数据进行代表性评价,以及利用有效等位基因、Nei’s遗传多样性指数、Shannon’s多样性指数等指标对核心种质的SSR分子标记数据进行代表性评价;并利用主成分分析对核心种质进行确认。结果表明,构建的甘薯核心种质包含289份材料,占全部种质的26.49%;在P<0.05概率下,核心种质中表型性状以及SSR分子标记的相关指标与全部种质无显著差异,且二者的表型频率分布基本一致;主成分分析表明核心种质具有与全部种质相似的遗传多样性和群体结构。建立的甘薯核心种质很好地代表了全部种质的遗传变异和群体结构,可为甘薯的品种改良、优良基因挖掘以及种质创新奠定良好基础。
To better preserve, study, and utilize sweet potato collection resources, 1091 sweetpotato germplasms preserved in the National Sweetpotato Germplasm Nursery(Guangzhou) were used as the materials in this study. Euclidean distance and Nei’s distance were used for NJ cluster grouping, respectively, and random sampling was conducted within the group to construct the core collection. Mean, variance, Shannon’s diversity index, coefficient of variation, and other indicators were used to evaluate the representativeness of the core collection based on phenotypic traits data, and effective alleles, Nei’s genetic diversity index,Shannon’s diversity index, and other indicators were used to evaluate the representativeness of the core collection based on SSR markers data. The results showed that the constructed sweetpotato core collection contained 289 materials, accounting for 26.49%of the entire collection. At P < 0.05, there was no significant difference in the related indicators of phenotypic traits and SSR molecular markers between the core collection and the entire collection, and the phenotypic frequency distribution of the two germplasm was basically the same. The principal component analysis revealed that the core collection had similar genetic diversity and population structure to the entire collection. In conclusion, the established core collection of sweetpotato well represented the entire collection’s genetic variation and population structure, which could lay a good foundation for variety improvement, excellent genes mining, and germplasm innovation of sweetpotato.
作者
陈伊航
唐朝臣
张雄坚
姚祝芳
江炳志
王章英
CHEN Yi-Hang;TANG Chao-Chen;ZHANG Xiong-Jian;YAO Zhu-Fang;JIANG Bing-Zhi;WANG Zhang-Ying(Crops Research Institute,Guangdong Academy of Agricultural Sciences/Guangdong Provincial Key Laboratory of Crop Genetic Improvement,Guangzhou 510640,Guangdong,China;College of Grassland Agriculture,Northwest A&F University,Yangling 712100,Shaanxi,China)
出处
《作物学报》
CAS
CSCD
北大核心
2023年第5期1249-1261,共13页
Acta Agronomica Sinica
基金
国家重点研发计划项目(2019YFD1000700,2019YFD1000701)
财政部和农业农村部国家现代农业产业技术体系建设专项
广东省甘薯马铃薯产业技术体系建设项目(2021KJ111)资助。
关键词
甘薯
核心种质
表型性状
SSR分子标记
种质资源
sweetpotato
core collection
phenotypic traits
SSR markers
germplasm resource
作者简介
通信作者:王章英,E-mail:wangzhangying@gdaas.cn;第一作者:陈伊航,联系方式:E-mail:chenyihang2022@126.com。
