摘要
目的探讨溃疡性结肠炎(UC)患者血清白细胞介素-1β(IL-1β)、可溶性白细胞介素-2受体(sIL-2R)、γ-干扰素(IFN-γ)水平与肠道菌群的关系。方法选取河南省驻马店市新蔡县人民医院2019年8月至2021年6月收治的158例UC患者作为研究对象,按病情严重程度将UC患者分为轻度组53例,中度组57例,重度组48例。另选取同期79例健康体检者作为对照组。比较入院时轻度组、中度组、重度组、对照组血清IFN-γ、IL-1β、sIL-2R水平及肠道菌群(双歧杆菌、乳酸杆菌、肠球菌、大肠杆菌)数。分析入院时血清IFN-γ、IL-1β、sIL-2R水平与肠道菌群数、UC病情严重程度的相关性。随访3个月后,根据UC复发情况将患者分为复发组、未复发组,比较上述两组入院2周时血清IFN-γ、IL-1β、sIL-2R水平及肠道菌群数。结果轻度组、中度组、重度组入院时血清IFN-γ、IL-1β、sIL-2R水平及肠球菌、大肠杆菌数均高于对照组,双歧杆菌、乳酸杆菌数均低于对照组,且随着UC严重程度的增加,血清IFN-γ、IL-1β、sIL-2R水平逐渐升高,肠球菌、大肠杆菌数逐渐增加,双歧杆菌、乳酸杆菌数逐渐减少,差异有统计学意义(P<0.05)。入院时UC患者血清IFN-γ、IL-1β、sIL-2R水平与双歧杆菌、乳酸杆菌数呈负相关(P<0.05),与肠球菌、大肠杆菌数及UC病情严重程度呈正相关(P<0.05)。与未复发组比较,复发组入院2周时血清IFN-γ、IL-1β、sIL-2R水平较高,双歧杆菌、乳酸杆菌数较少,肠球菌、大肠杆菌数较多,差异有统计学意义(P<0.05)。结论UC患者血清IFN-γ、IL-1β、sIL-2R水平与肠道菌群数具有相关性,临床可通过检测上述指标评估患者预后情况,以制订针对性的治疗方案,降低复发率。
Objective To investigate the relationship between serum levels of interleukin-1β(IL-1β),soluble interleukin-2 receptor(sIL-2R),γ-interferon(IFN-γ)and intestinal flora in patients with ulcerative colitis(UC).Methods A total of 158 UC patients admitted to Xincai County People′s Hospital from August 2019 to June 2021 were selected as research objects.According to the severity of UC,the patients were divided into mild group(53 cases),moderate group(57 cases)and severe group(48 cases).Another 79 healthy people underwent physical examination in the same period were selected as control group.The serum levels of IFN-γ,IL-1βand sIL-2R and the number of intestinal flora(Bifidobacterium,Lactobacillus,Enterococcus and Escherichia coli)in mild group,moderate group,severe group and control group were compared at admission.The correlation of serum levels of IFN-γ,IL-1βand sIL-2R with the number of intestinal flora and the severity of UC was analyzed.After 3 months of follow-up,patients were divided into recurrence group and non-recurrence group according to UC recurrence situation,and serum levels of IFN-γ,IL-1β,sIL-2R and the number of intestinal flora in the two groups were compared at 2 weeks after admission.Results The levels of serum IFN-γ,IL-1βand sIL-2R and the numbers of Enterococcus and Escherichia coli in mild,moderate and severe groups were higher than those in control group,while the numbers of Bifidobacterium and Lactobacillus were lower than those in control group,with the increased of the severity of UC,the serum levels of IFN-γ,IL-1β,sIL-2R and the numbers of Enterococcus and Escherichia coli were gradually increased,while the numbers of Bifidobacterium and Lactobacillus were gradually decreased,the differences were statistically significant(P<0.05).The serum levels of IFN-γ,IL-1βand sIL-2R in UC patients at admission were negatively correlated with the numbers of Bifidobacterium and Lactobacillus(P<0.05),and positively correlated with the numbers of Enterococcus and Escherichia coli and the severity of UC(P<0.05).Compared with the non-recurrence group,the serum levels of IFN-γ,IL-1βand sIL-2R in the recurrence group were higher,the numbers of Bifidobacterium and Lactobacillus were less,and the numbers of Enterococcus and Escherichia coli were more at 2 weeks after admission,the differences were statistically significant(P<0.05).Conclusion The serum levels of IFN-γ,IL-1βand sIL-2R in UC patients are correlated with the number of intestinal flora.The above indicators could be detected to evaluate the prognosis of patients in clinical practice,so as to formulate targeted treatment plans and reduce the recurrence rate.
作者
徐素芳
张英
赵小燕
XU Sufang;ZHANG Ying;ZHAO Xiaoyan(Department of Clinical Laboratory,Xincai County People′s Hospital,Zhumadian,Henan 463500,China;Department of Clinical Laboratory,Henan Provincial People′s Hospital,Zhengzhou,Henan 450000,China;Department of Clinical Laboratory,Zhumadian Central Hospital,Zhumadian,Henan 463000,China)
出处
《检验医学与临床》
CAS
2023年第5期664-667,672,共5页
Laboratory Medicine and Clinic
作者简介
徐素芳,女,主管技师,主要从事临床医学检验技术方面的研究。
