摘要
目的研究宽叶重楼根茎的甾体皂苷类成分。方法采用硅胶柱色谱、Sephadex LH-20凝胶柱色谱、中压制备液相色谱和半制备高效液相色谱等方法,从宽叶重楼根茎70%乙醇提取物的正丁醇萃取部位分离化合物,通过波谱分析和化学手段鉴定化学结构。结果分离得到7个甾体皂苷类化合物,分别鉴定为偏诺皂苷元-3-O-α-L-吡喃鼠李糖基-(1→4)-[α-L-吡喃鼠李糖基-(1→2)]-β-D-吡喃葡萄糖苷(1)、17-hydroxygracillin(2)、重楼皂苷H(3)、重楼皂苷Ⅶ(4)、薯蓣皂苷元-3-O-α-L-呋喃阿拉伯糖基-(1→4)-β-D-吡喃葡萄糖苷(5)、重楼皂苷Ⅴ(6)、重楼皂苷Ⅱ(7)。结论化合物2、3、5和6为首次从宽叶重楼中分离得到。
Objective To determine the saponin constituents from rhizomes of Paris polyphylla var.latifolia.Methods The compounds in the n-butanol fraction of 70%ethanol extract of rhizomes of Paris polyphylla var.latifolia were isolated and purified by silica gel,Sephadex LH-20,middle-pressure liquid chromatography and semi-preparative high pressure liquid chromatography.Their structures were identified by spectroscopic analysis and chemical methods.Results Totally 7 steroidal saponins were isolated and identified as pennogenin-3-O-α-L-rhamnopyranosyl-(1→4)-[α-L-rhamnopyranosyl-(1→2)]-β-D-glucopyranoside(1),17-hydroxygracillin(2),paris saponin H(3),polyphyllinⅦ(4),diosgenin-3-O-α-L-arabinofuranosyl-(1→4)-β-D-glucopyranoside(5),polyphyllinⅤ(6),and polyphyllinⅡ(7).Conclusion Compounds 2,3,5 and 6 have been isolated from this plant for the first time.
作者
李天怡
陆云阳
刘杨
曹宇
陈文文
田韵远
胡晋铭
汤海峰
LI Tian-yi;LU Yun-yang;LIU Yang;CAO Yu;CHEN Wen-wen;TIAN Yun-yuan;HU Jin-ming;TANG Hai-feng(Department of Chinese Materia Medica and Natural Medicines,School of Pharmacy,Air Force Medical University,Xi’an 710032)
出处
《中南药学》
CAS
2022年第12期2715-2721,共7页
Central South Pharmacy
基金
国家自然科学基金面上项目(No.81973192)。
关键词
宽叶重楼
甾体皂苷
化学成分
提取分离
结构鉴定
Paris polyphylla var.latifolia
steroidal saponin
chemical constituent
extraction and isolation
structure elucidation
作者简介
李天怡,女,硕士研究生,主要从事天然药物化学研究,email:suchacuteguy@163.com;通信作者:汤海峰,男,主任药师,博士研究生导师,主要从事天然药化研究和中药、海洋药物开发,email:tanghaifeng71@163.com。
