摘要
The Shack-Hartmann wavefront sensor(SHWS)is an essential tool for wavefront sensing in adaptive optical microscopes.However,the distorted spots induced by the complex wavefront challenge its detection performance.Here,we propose a deep learning based wavefront detection method which combines point spread function image based Zernike coefficient estimation and wavefront stitching.Rather than using the centroid displacements of each micro-lens,this method first estimates the Zernike coefficients of local wavefront distribution over each micro-lens and then stitches the local wavefronts for reconstruction.The proposed method can offer low root mean square wavefront errors and high accuracy for complex wavefront detection,and has potential to be applied in adaptive optical microscopes.
基金
Project supported by the National Natural Science Foundation of China(Nos.61735016,81771877,and 61975178)
the Zhejiang Provincial Natural Science Foundation of China(No.LR20F050002)
the Key R&D Program of Zhejiang Province,China(No.2021C03001)
the CAMS Innovation Fund for Medical Sciences,China(No.2019-I2M-5-057)
the Fundamental Research Funds for the Central Universities,China。
作者简介
Shuwen HU,first author of this invited paper,received her BS degree from Tianjin University,China.She is currently a master degree candidate at the College of Optical Science and Engineering,Zhejiang University,China.Her research interests include adaptive optics,machine learning,and deep tissue imaging.contributed equally to this work;Lejia HU,contributed equally to this work;Corresponding authors:Wei GONG,corresponding author of this invited paper,is a PI at Zhejiang University School of Medicine,China.She received her BS and MS degrees from Zhejiang University,China,and her PhD degree from the National University of Singapore,Singapore.She is a special expert of the Ministry of Education(MOE)and the Outstanding Youth of Zhejiang Province.Her research interests include biomedical imaging,optical clearing,and artificial intelligence in biomedicine.E-mail:weigong@zju.edu.cn,https://orcid.org/0000-0003-3969-5607;Corresponding authors:Ke SI,corresponding author of this invited paper,is a professor at the College of Optical Science and Engineering,Zhejiang University,China,and a joint professor at Zhejiang University School of Medicine.He is the Vice Director of MOE Frontier Science Center for Brain Science and Brain-Machine Integration,and the Vice Dean of the School of Brain Science and Brain Medicine.He is now a corresponding expert of Front Inform Technol Electron Eng.His research focuses on biophotonics,deep tissue imaging,adaptive optics,and optogenetics.kesi@zju.edu.cn,https://orcid.org/0000-0001-8328-4325。
