摘要
为了分析种植密度对地黄发育、品质及基因转录特性的影响,以85-5和J9为材料,设置了5000、25000、50000株/亩(1亩≈667 m^(2))3个种植密度,分析了地黄叶片和块根的农艺性状,梓醇和毛蕊花糖苷的含量,以及基因表达的变化规律。结果表明,低密度下地黄的叶片大小、单株叶生物量、块根直径、单株块根数、单株块根生物量均明显高于中、高密度。高密度下叶中的梓醇和毛蕊花糖苷含量均较高。块根中的梓醇含量在低密度下较高,变化趋势与叶中类似;而块根中的毛蕊花糖苷含量在高密度下较高。转录组分析发现约1/2的扩展蛋白基因能够响应密度的处理而呈规律性变化,与块根的发育有关。参与梓醇和毛蕊花糖苷生物合成的多个催化酶基因的表达量变化趋势与它们含量的变化趋势一致,可能参与了密度响应的药效成分积累调控。该研究基于大田栽培条件下不同种植密度的地黄发育、药效成分含量和基因表达特征的分析,对通过管理地黄种植密度调控地黄药材的品质和产量提供了重要的依据。
The present study analyzed the effects of planting density on the development,quality,and gene transcription characte-ristics of Rehmannia glutinosa using 85-5 and J9 as materials with three planting densities of 5000,25000,and 50000 plants/Mu(1 Mu≈667 m^(2)).The agronomic characteristics of leaves and tuberous roots,the content of catalpol and acteoside,and the changes of gene expression were determined.The results showed that the leaf size,the diameter of tuberous root,leaf biomass,tuberous root number,and tuberous root biomass per plant at low density were significantly higher than those of medium and high densities.The content of catalpol and acteoside in leaves was higher at high density.The content of catalpol in tuberous roots was higher at low density,and the change trend was similar to that in leaves,while the content of acteoside in tuberous roots was higher at high density.Transcriptome analysis found that about 1/2 of the expansin genes could change regularly in response to density treatment,which was rela-ted to the development of tuberous roots.The change trend of the gene expression of multiple catalytic enzymes involved in the biosynthesis of catalpol and acteoside was consistent with that of their content,which was presumedly involved in the accumulation and regulation of density-responsive medicinal components.Based on the analysis of the development,medicinal components,and gene expression characteristics of R.glutinosa at different densities,this study is expected to provide an important basis for regulating the quality and yield of medicinal materials of R.glutinosa by managing the planting density.
作者
王丰青
杨超飞
李铭铭
左鑫
杨旭
李欣容
古力
杜家方
李明杰
王浩
张重义
WANG Feng-qing;YANG Chao-fei;LI Ming-ming;ZUO Xin;YANG Xu;LI Xin-rong;GU Li;DU Jia-fang;LI Ming-jie;WANG Hao;ZHANG Zhong-yi(College of Agronomy,Henan Agricultural University,Zhengzhou 450002,China;College of Agriculture,Fujian Agriculture and Forestry University,Fuzhou 350002,China)
出处
《中国中药杂志》
CAS
CSCD
北大核心
2021年第17期4367-4379,共13页
China Journal of Chinese Materia Medica
基金
国家自然科学基金项目(81872950,81473299)
国家重点研发计划项目(2017YFC1700705)
现代农业产业技术体系建设专项(CARS-21)。
关键词
地黄
密度
生长发育
质量
基因表达
Rehmannia glutinosa
density
growth and development
quality
gene expression
作者简介
通信作者:杜家方,博士,主要从事中药资源学研究,E-mail:dujiafang@henaii.edu.cn;通信作者:张重义,博士,教授,主要从事中药资源生态与中药材生产技术研究,E-mail:zyzhang@fafu.edu.cn;王丰青,博士,副教授,E-mail:zyzhang@fafu.edu.cn。
