摘要
【目的】开展思茅松花粉活力研究,为思茅松杂交育种提供技术保障。【方法】以思茅松花粉为研究对象,研究了蔗糖、硼酸和氯化钙单因子及正交试验对思茅松花粉萌发和花粉管生长的影响。【结果】培养基内蔗糖、硼酸和氯化钙在一定质量浓度范围内,对花粉萌发及花粉管生长起促进作用,花粉生活力测定最适蔗糖、硼酸和氯化钙用量分别为10%、100 mg/L和100 mg/L;正交试验中蔗糖对思茅松花粉萌发和花粉管生长有显著影响,最佳配方组合为10%蔗糖+30 mg/L硼酸+100 mg/L氯化钙。【结论】获得了适宜思茅松花粉活力检测的离体培养基。
[Purpose]The pollen viability was studied in order to provide the technical support for cross breeding of Pinus kesiya var.langbianensis.[Method]The effects of sucrose,H3BO3 and CaCl2 single factor and orthogonal test on the pollen germination and pollen tube growth of P.kesiya var.langbianensis in vitro were studied.[Results]The pollen germination and pollen tube growth would be enhanced when the concentration of sucrose,H3BO3 and CaCl2 of the culture medium were within a certain range.The single suitable concentration of sucrose,H3BO3 and CaCl2 for pollen viability test were 10%,100 mg/L and 100 mg/L,respectively.Sucrose had significant effect on the pollen germination and pollen tube growth in orthogonal test.The optimum culture medium which support the germination and tube growth was 10%sucrose+30 mg/L H3BO3+100 mg/L CaCl2.[Conclusion]The culture medium suitable for detection of pollen viability of P.kesiya var.langbianensis was obtained.
作者
陈伟
李江
孟梦
陈绍安
冯弦
CHEN Wei;LI Jiang;MENG Meng;CHEN Shao'an;FENG Xian(Yunnan Provincial Key Laboratory of Cultivation and Exploitation of Forest Plants,Kunming 650201 China;Yunnan Academy of Forestry and Grassland,Kunming 650201 China)
出处
《云南农业大学学报（自然科学版）》
CSCD
北大核心
2020年第3期496-501,共6页
Journal of Yunnan Agricultural University:Natural Science
基金
国家重点研发计划子课题(2017YFD0600504-3)。
关键词
思茅松
花粉
离体萌发
花粉管生长
Pinus kesiya var.langbianensis
pollen
germination in vitro
pollen tube growth
作者简介
陈伟(1982-),男,云南宁洱人,硕士,副研究员,主要从事森林培育研究。E-mail:chenwei176@126.com;通信作者:李江(1972-),男,云南昌宁人,博士,研究员,主要从事林业碳汇和森林培育研究。E-mail:lijianglyht@163.com。
