摘要
目的探究大豆苷元(daidzein,Daid)对非小细胞性肺癌细胞株A549和H1299增殖、迁移能力的影响及可能机制。方法以A549和H1299为研究对象,CCK-8法检测Daid(0,5,10,25,50,100,200μmol·L^-1)抑制A549和H1299两种细胞的增殖情况。划痕和Transwell小室实验检测Daid对肺癌细胞迁移和侵袭能力的影响。Western blot检测Cleaved Caspase-3和LC3Ⅱ/Ⅰ等的蛋白表达。结果与对照组相比,Daid在体外可明显抑制肺癌细胞的增殖,且抑制作用呈浓度依赖关系。Daid(100μmol·L^-1)可明显降低人肺癌细胞A549和H1299的迁移侵袭能力。Western blot结果显示,Daid(100μmol·L^-1)预处理后,两种肺癌细胞的Cleaved Caspase-3及LC3Ⅱ/Ⅰ表达升高。结论Daid可抑制人非小细胞肺癌A549和H1299细胞的增殖,使上述细胞的迁移侵袭能力明显下降,其机制可能与Daid诱导细胞凋亡及自噬有关。
Aim To investigate the effect of Daidzein(Daid)on proliferation and migration of non-small cell lung cancer cell lines A549 and H1299 and its possible mechanism.Methods CCK-8 was adopted to detect cell proliferation of A549 and H1299 cells inhibited by Daid(0,5,10,25,50,100,200μmol·L^-1).The effect of Daid on proliferation and migration of lung cancer cells was detected by scratch healing and Transwell chamber assay.The protein expressions of cleaved Caspase-3 and LC3Ⅱ/Ⅰwere detected by Western blot.Results Daid had significantly inhibitory effects on the proliferation of lung cancer cells in vitro in a concentration-dependent manner when compared with control group.Daid(100μmol·L^-1)could inhibit the migration and invasion ability of human lung cancer cells A549 and H1299.Western blot results showed that the expressions of cleaved Caspase-3 and LC3Ⅱ/Ⅰwere elevated after Daid pretreatment.Conclusions Daid can inhibit the proliferation of human non-small cell lung cancer cells A549 and H1299,and reduce the migration and invasion of cells.The mechanism may be related to cell apoptosis and autophagy induced by Daid.
作者
成琼
李真
陈锦辉
向铮
张献伟
孔令非
CHENG Qiong;LI Zhen;CHEN Jin-hui;XIANG Zheng;ZHANG Xian-wei;KONG Ling-fei(Dept of Pathology,Henan Provincial People’s Hospital,People’s Hospital of Zhengzhou University,Zhengzhou 450003,China)
出处
《中国药理学通报》
CAS
CSCD
北大核心
2020年第2期245-249,共5页
Chinese Pharmacological Bulletin
基金
河南省重大科技专项(No 161100311400)
河南省医学科技攻关计划联合共建项目(No 2018020400)。
作者简介
成琼(1980-),女,博士,主治医师,研究方向:肿瘤的分子病理诊断及生物靶向治疗,E-mail:chengqiong2008@126.com;通讯作者:孔令非(1963-),男,主任医师,教授,硕士生导师,研究方向:肿瘤的分子病理诊断及生物靶向治疗,E-mail:lfkong9@163.com。
