摘要
为研究饲料n-3/n-6高不饱和脂肪酸(HUFA)对许氏平鲉幼鱼生长、体组成及组织脂肪酸组成的影响,配制了6种n-3/n-6 HUFA(D1:14.28,D2:9.26,D3:5.66,D4:3.06,D5:2.02,D6:1.50)的等氮等脂的实验饲料。以许氏平鲉幼鱼(36.30±0.03) g为研究对象,在网箱中养殖65 d,分为6实验组,每组设3个重复,每个重复30尾鱼。结果发现:①饲料n-3/n-6 HUFA对许氏平鲉幼鱼的成活率无显著影响。随着n-3/n-6 HUFA降低,幼鱼增重率呈先上升后下降趋势,饲料系数呈相反趋势,D2和D3组的增重率显著高于各组。②全鱼和肌肉粗脂肪含量呈先上升后下降趋势,分别在D2、D3组达到最大值。肝脏粗脂肪含量呈先下降后上升趋势,D2组显著小于其他各组。③各组织C20:4n-6含量随n-3/n-6 HUFA的降低均呈上升趋势,而C20:5n-3、C22:6n-3和n-3/n-6 HUFA整体呈下降趋势。④鱼体脂肪酸组成受饲料影响程度由大到小依次为腹脂、肌肉、全鱼、肝脏,且各组织C20:5n-3与饲料C20:4n-6均呈显著负相关。研究表明,在本实验条件下,饲料中适宜比例(5.66~9.26)的n-3/n-6 HUFA显著提高实验鱼的生长,改变体组成及组织脂肪酸组成,以增重率和饲料系数作评价指标,经一元二次回归分析得许氏平鲉幼鱼饲料中n-3/n-6 HUFA的适宜比例分别是8.93和8.70。
A 65 d feeding trial was conducted in a float cage to study the effect of dietary n-3/n-6 HUFA(D1:14.28, D2:9.26, D3:5.66, D4:3.06, D5:2.02, D6:1.50) on growth, body composition and fatty acid composition of tissue in juvenile rockfish (Sebastes schlegeli). Six diets were formulated to feed six groups of juvenile S. schlegeli [mean initial weight (36.30±0.03) g], respectively. Each diet was randomly fed to triplicate groups of 30 fish per cage. The results show that:①Dietary n-3/n-6 HUFA had no significant impact on the survival rate of fish (P>0.05).With the decreasing of dietary n-3/n-6 HUFA, the weight gain rate first increased and then decreased, the feed conversion ratio (FCR) showed an opposite trend, and the weight gain rate of D2 and D3 groups was significantly higher than that of other groups (P<0.05).②The crude fat in the whole fish and muscle showed a trend of increased first and then decreased, which reaching their peaks in group D2 and D3, respectively(P<0.05).The crude fat in the liver first decreased and then increased, and the D2 group was significantly lower than the other groups (P<0.05).③ The content of C20:4n-6 in all tissues were increased with the decreasing of dietary n-3/n-6 HUFA. C20:5n-3, C22:6n-3 and n-3/n-6 HUFA in all tissues showed an overall decreased trend.④The fatty acid composition of fish body was affected by the dietary, in order of abdominal fat, muscle, whole fish and liver. The dietary C20:4n-6 was negatively correlated with C20:5n-3 of all tissues. Under this experimental conditions, the appropriate proportion of dietary n-3/n-6HUFA (5.66-9.26) can significantly improve the growth of experimental fish, and modify body composition and fatty acid composition of tissues. The univariate quadratic regression analysis, based on weight gain rate and feed conversion ratio, showed that the appropriate proportion of n-3/n-6 HUFA in the dietary of juvenile S. schlegeli is 8.93 and 8.70, respectively.
作者
马长兴
王际英
李宝山
王成强
沈钰博
刘财礼
周莹
MA Changxing;WANG Jiying;LI Baoshan;WANG Chengqiang;SHEN Yubo;LIU Caili;ZHOU Ying(National Demonstration Center for Experimental Fisheries Science Education,Centre for Research on Environmental Ecology andFish Nutrition (CREEFN) of the Ministry of Agriculture,Shanghai Collaborative Innovation for Aquatic Animal Genetics and Breedin,College of Fisheries and Life Science,Shanghai Ocean University,Shanghai 201306,China;Shandong Key Laboratory of Marine Ecological Restoration,Shandong Marine Resource and Environment Research Institute,Yantai 264006,China)
出处
《水产学报》
CAS
CSCD
北大核心
2019年第10期2138-2153,共16页
Journal of Fisheries of China
基金
烟台市科技计划(2017ZH068)~~
作者简介
通信作者:王际英,E-mail:ytwjy@126.com.
