摘要
为探寻墨兰瓶内诱导假鳞茎形成和生长膨大的最佳配方组合,以墨兰组培苗为材料,研究不同基础培养基种类、蔗糖浓度、pH值、激素组合对兰属墨兰假鳞茎形成和生长的影响。结果表明:诱导墨兰假鳞茎形成和促进其生长的最佳基本培养基为N6、蔗糖浓度30g·L^-1、pH5.2、激素组合6BA0.1mg·L^-1+ZT0.5mg·L^-1+NAA0.5mg·L^-1。该研究结果可促进墨兰组培苗假鳞茎的形成及生长,提高幼苗成活率、缩短种苗栽培时间。
In order to explore the best formula combination for inducing the formation and growth expansion of Cymbidium sinense in the bottle, the effects of different types of basal culture medium, sucrose concentration, pH value and hormone combination on the formation and growth of Cymbidium sinense were studied. The results showed that the optimal formula combination for inducing the formation and growth of Cymbidium sinense pseudobulb was that basic culture medium of N6, sucrose concentration of 30 g·L ^-1 , pH value of 5.2, and hormone combination of 6 BA 0.1 mg·L^-1 +ZT 0.5 mg·L^-1 +NAA 0.5 mg·L^-1 . The results could promote the formation and growth of tissue culture seedling pseudobulb of Cymbidium sinense , improve the survival rate of seedlings and shorten the cultivation time of seedlings.
作者
林辉锋
莫智龙
陈昌铭
陈允浩
宋彩凤
周辉明
LIN Hui-feng;MO Zhi-long;CHEN Chang-ming;CHEN Yun-hao;SONG Cai-feng;ZHOU Hui-ming(Sanming Academy of Agricultural Sciences,Sanming,Fujian 365051,China;Ningde Agricultural Service Center in Huotong Town of Jiaocheng District,Ningde,Fujian 352112,China;Sanming Sencai Ecological Agriculture Development Co.,LTD.,Sanming,Fujian 365051,China)
出处
《福建农业科技》
2019年第8期23-27,共5页
Fujian Agricultural Science and Technology
基金
福建省科技星火计划项目(2018S0036)
关键词
墨兰
假鳞茎
诱导
生长
培养基
Cymbidium sinense
Pseudobulb
Induce
Growth
Culture medium
作者简介
林辉锋,男,1984年生,助理研究员,主要从事植物组织培养研究;通信作者:周辉明,男,1980年生,副研究员,主要从事植物组织培养研究(E-mail:hmzhou1980@163.com)。
