摘要
目的:探讨中药太灵丹对链脲佐菌素(STZ)诱导糖尿病肾病大鼠肾脏保护作用的影响。方法:选择70只Wistar大鼠,根据体质量随机分为正常对照组10只,其余60只采用STZ诱导为糖尿病肾病大鼠模型,并分为模型组,太灵丹低剂量组[生药5 g/(kg·d)灌胃]、中剂量组[生药10 g/(kg·d)灌胃]、高剂量组[生药20 g/(kg·d)灌胃]及缬沙坦组[缬沙坦10 mg/(kg·d)灌胃],每组12只。连续处理14周,检测各组大鼠体质量、血糖、糖化血红蛋白(HAblc)、24小时尿蛋白定量、血肌酐(Scr)、尿素氮(BUN)及甘油三酯(TG)的变化。用HE、PAS及Masson染色法观察肾脏病理改变。用免疫组织化学法检测肾组织转化生长因子β1 (TGF-β1)、E-钙黏蛋白(E-cadherin)及α-平滑肌肌动蛋白(α-SMA)的表达。结果:1)模型组血糖、HAb1c、24小时尿蛋白定量指标均较正常对照组大鼠明显升高(P<0.01或0.05)。病理学观察模型组大鼠肾小球肥大,基底膜增厚,细胞外基质增多及肾小管上皮细胞大量萎缩、空泡变性,间质内炎性细胞浸润。太灵丹各剂量组、缬沙坦组,上述改变较模型组明显减轻(P<0.05),但组间比较差异无统计学意义(P>0.05)。2)免疫组化显示:与正常对照组比较,模型组肾小管上皮细胞中E-cadherin表达减弱(P<0.05),TGF-β1及α-SMA表达增强(P<0.01)。太灵丹各剂量组及缬沙坦组,E-cadherin表达增强(P<0.05),TGF-β1及α-SMA表达减弱(P<0.05),但组间比较差异无统计学意义(P>0.05)。结论:太灵丹可能通过增强E-cadherin、减弱TGF-β1及α-SMA的表达改善肾小管上皮细胞转分化,从而减缓糖尿病肾间质纤维化的进展。
Objective:To discuss the effects of TaiLing pills on renal protection of the rats with STZ-induced diabetic nephropathy.Methods:Seventy Wistar rats were allocated to ten rats in normal control group and other 60 rats,which were induced into the models suffering diabetic nephropathy by STZ,and separated into the model group,low[intragastric administration of crude drug,5g/(kg·d)],moderate[intragastric administration of crude drug,10g/(kg·d)]and high[intragastric administration of crude drug,20g/(kg·d)]dosages groups of TaiLing pills and valsartan group[intragastric administration of valsartan,10mg/(kg·d)],12 rats each group.The changes of triglyceride(TG),blood usea nitrogen(BUN),serum creatinine(Scr),24h urinary protein quantification,glycosylated hemoglobin(HAb1c),blood glucose and body mass were detected after the rats were handled consecutively for 14 weeks.Pathological changes of the kidney were observed by HE,PAS and Masson staining method,and the expressions ofα-Smooth muscle actin(α-SMA),E-cadherin and TGF-β1 in renal tissue were measured by immunoh-istochemical method.Results:1)Blood glucose,HAb1c and the indexes of 24h urinary protein quantification of the rats in the model group rose obviously compared with normal control group(P<0.01 or 0.05).Pathological results showed that glomerular hypertrophy,basement membrane thickening,extracellular matrix increased and renal tubular epithelial cell shrank largely,vacuolar degeneration,interstitial inflammatory cells infiltrated in the model group.The changes above in different dosages groups of TaiLing pills and valsartan group relieved notably compared with the model group(P<0.05),but the differences had no statistical meaning in the comparisons among the groups(P >0.05).2)Immunohistochemical results displayed that:compared with normal control group,the expressions of E-cadherin in renal tubular epithelial cells reduced in the model group(P<0.05),the expressions of TGF-β1 andα-SMA enhanced(P<0.01).The expressions of E-cadherin strengthened,the expressions of TGF-β1 andα-SMA reduced(P<0.05)in different dosages groups of TaiLing pills and valsartan group,while the difference had no statistical meaning in the comparisons among the groups(P >0.05).Conclusion:TaiLing pills could improve renal tubular epithelial cell trans-differentiation by enhancing the expressions of E-cadherin,reducing the expressions of TGF-β1 andα-SMA,therefore to delay the progression of interstitial fibrosis of DN patients.
作者
李敏州
武忠
张瑞霞
高巍
赵凯莹
LI Minzhou;WU Zhong;ZHANG Ruixia;GAO Wei;ZHAO Kaiying(TCM Department,Inner Mongolia People's Hospital,Hohehot 010017,China)
出处
《西部中医药》
2018年第12期5-9,共5页
Western Journal of Traditional Chinese Medicine
基金
内蒙古自治区自然科学基金项目(编号2014BS0810)
内蒙古自治区人民医院博士科研启动资金(编号BS201516)
关键词
糖尿病肾病
太灵丹
肾小管上皮细胞转分化
肾脏纤维化
动物实验
diabetic nephropathy
TaiLing pills
tubular epithelial mesenchymal trans-differentiation
renal fibrosis
animal experiments
作者简介
李敏州(1983—),女,博士学位,副主任医师。研究方向:中医药防治糖尿病及其并发症。
