摘要
选取血红密孔菌Pycnoporus coccineus为产漆酶菌种,通过开展单因素产漆酶条件优化研究,明确最佳液体发酵工艺参数,制备出粗酶制剂并进行原油降解性能评价。结果表明:1)漆酶最佳液体发酵工艺参数为初始pH值5～7、初始温度30℃、摇床转速200r/min,接种量1.5%、碳源为羧甲基纤维素钠20.00g/L、氮源为硝酸钠5.00g/L、磷源磷酸氢二钾为2.00g/L、Cu2+浓度为1.00mmol/L、诱导剂为蔥2.00×10-4 mmol/L;复合制剂(粗酶制剂+菌剂)TPHs降解率高于单一制剂(仅投加菌液),环境耐受性、微生物底物作用能力及原油降解速率均显著提升。
Pycnoporuscoccineus was selected as the laccase producing strain.By optimizing the conditions of single factor laccase production,the optimal liquid fermentation process parameters were determined,and the crude enzyme was prepared and the degradation performance of crude oil was evaluated.The results show that:1)the optimum liquid fermentation process parameters for laccase are initial pH was between 5 and 7;the initial temperature was 30℃;the shaking speed was 200 r/min;the inoculum was 1.5%(V/V);the alternative carbon source was carboxymethyl cellulose sodium(20.00 g/L);the alternative nitrogen source was NaNO 3(5.00 g/L);the alternative phosphorus source was KH 2PO 3(2.00 g/L);the Cu^2+concentration was 1.00 mmol/L;add inducer was shallot(2.00×10^-4 mmol/L);2)the degradation rate of TPHs in the composite preparation(crude enzyme+bacterial agent)was higher than the single preparation(bacterial agent),and the environmental tolerance,the microbial substrate action ability and the crude oil degradation rate were all significantly improved.
作者
宋佳宇
李兴春
于文赫
吴百春
Song Jiayu;Li Xingchun;Yu Wenhe;Wu Baichun(State Key Laboratory of Petroleum Pollution Control,Beijing,102206,China;CNPCResearch Institute of Safety and Environmental Technology,Beijing,102206,China)
出处
《绿色科技》
2019年第2期45-49,共5页
Journal of Green Science and Technology
基金
中国石油天然气集团公司科学研究与技术开发项目(编号:2016D-5006-08)
关键词
石油污染修复
漆酶
血红密孔菌
产酶条件优化
性能评价
oil pollution remediation
laccase
Pycnoporuscoccineus
optimization of enzyme production conditions
performance evaluation
作者简介
宋佳宇(1985-),女,硕士,高级工程师,研究方向为石油污染生物修复。
