摘要
以铁皮石斛种子为起始材料,研究了其原球茎萌发、壮苗、生根出苗环节中一些关键影响因子,建立了铁皮石斛种子快速成苗组培技术体系。研究发现,培养基中加入6-BA会抑制原球茎的发育,若整个培养阶段用直径为9 cm的培养皿,起始播种密度以8 000粒种子为宜,冻干香蕉粉对于种子的萌发是必需的,以5～10 g·L^(-1)为宜;壮苗阶段,接种密度以100～120株·瓶-1为宜,培养基及光质对铁皮石斛壮苗影响远小于接种密度;生根阶段,利用3 000 K LED白光代替荧光灯照明,在不降低组培苗茎粗的前提下,可有效提高组培苗株高,增加优质苗比例。研究结果还表明,冻干香蕉粉可完全代替剥皮香蕉作为有机添加物用于铁皮石斛组培苗的培养。整个培养过程中,培养温度(24±2)℃,种子萌发阶段光照时间10 h,光照强度25～30μmol·m^(-2)·s^(-1);其余培养阶段均光照时间12 h,光照强度40μmol·m^(-2)·s^(-1)左右。
Some key factors affecting the seed germination,plant growing and rooting of Dendrobium officinale were studied,and the micropropagation system of Dendrobium officinale via seed culture was established.The results showed that 6-benzyladenine(6-BA)in the medium inhibited the development of the protocorm seriously.The suitable seed density for initial germination was 8 000 in the condition that the culture vessel with a diameter of 9 cm was used,and banana powder as organic additives was necessary for seed germination with the suitable amount of 5-10 g·L^-1.The inoculation density was suitable for 100-120 plantlets per bottle in plant growth stage,and inoculation density played a more important role than light quality and medium content.In rooting stage,the plantlet height could be improved greatly when cultured in 3 000 K LED white light than those in fluorescent lamp without reducing the stem diameter,and the proportion of high quality seedlings also improved a lot.The results also showed that banana powder could completely replace fresh banana as organic additives for tissue culture of Dendrobium candidum.During the whole culture process,the temperature was(24±2)℃.The photoperiod for seed germination was 10 h and the light intensity was 25-30μmol·m^-2·s^-1,while in the other two stages,the photoperiod kept at 12 h and the light intensity kept around 40μmol·m^-2·s^-1 were enough.
作者
陈志
汪一婷
吕永平
牟豪杰
陈剑平
CHEN Zhi;WANG Yiting;LYU Yongping;MU Haojie;CHEN Jianping(Institute of Virology and Biotechnology,Zhejiang Academy of Agricultural Sciences,Hangzhou 310021,China;Institute of Plant Virology,Ningbo University,Ningbo 315211,China)
出处
《浙江农业学报》
CSCD
北大核心
2018年第11期1870-1878,共9页
Acta Agriculturae Zhejiangensis
基金
原农业部"引进国际先进农业技术"(2011-G31
2016-X23)
关键词
铁皮石斛
组织培养
播种
优化
Dendrobium officinale
tissue culture
seed culture
optimization
作者简介
陈志(1978—),男,湖北恩施人,博士,助理研究员,主要从事植物组织培养相关研究。E-mail:czdmh@163.com;通信作者,陈剑平,E-mail:jpchen2001@126.com。
