摘要
目的:探讨葡萄糖调节蛋白78(GRP78)对前列腺癌细胞增殖的调控作用及机制。方法:采用免疫组织化学染色检测60例前列腺癌和40例40例良性前列腺增生组织GRP78表达;利用反义核苷酸技术下调PC-3细胞GRP78表达,CCK-8检测细胞增殖能力,流式细胞术检测细胞凋亡率,Western blot检测线粒体凋亡通路相关蛋白的表达。结果:前列腺增生组织GRP78蛋白阳性率(86.7%,52/60)明显高于前列腺增生组织(35.0%,14/60)(χ2=28.550,P<0.000 1)。转染组在24 h、48 h、72 h后增殖能力明显低于阴性对照组和空白对照组(P<0.05),阴性对照组和空白对照组细胞增殖能力比较差异无统计学意义(P>0.05)。与阴性对照组和空白对照组比较,转染组细胞凋亡率明显增加(P<0.05),阴性对照组和空白对照组细胞凋亡率比较差异无统计学意义(P>0.05)。与阴性对照组和空白对照组比较,转染组细胞Caspase-9、Bax蛋白表达明显增加,Bcl-2蛋白表达明显减少(P<0.05),阴性对照组和空白对照组Caspase-9、Bcl-2、Bax蛋白表达比较差异无统计学意义(P>0.05)。结论:下调GRP78表达可以抑制前列腺癌细胞的增殖和促进细胞凋亡,GRP78可能通过线粒体凋亡通路调控前列腺癌细胞的增殖。
Objective：To explore the effect of glucose regulated protein 78（GRP78）on prostatic carcinoma cell growth and its mechanism.Methods：The expression of GRP78 in sixty prostatic carcinoma patients and forty benign prostatic hyperplasia patients tissues was measured by immunohistochemistry assay,and the expression of GRP78 was down-regulated by antisense nucleotide technology,the ability of cell proliferation was measured by CCK-8 assay,the cell apoptosis was measured by flow cytometry,the relative protein expression of mitochondrial apoptotic pathway was performed by Western blot.Results：The positive rate of GRP78 protein in the prostatic carcinoma group（86.7%,52/60）was significantly lower than that in the BPH group（35.0%,14/60）（χ2=28.550,P 〈 0.0001）.After 24 h,48 h,72 h of transfection,the cell proliferation ability in the transfection group was significantly lower than that in the negative group and the blank control group（P 〈 0.05）,while there was no significant difference of proliferation between the negative group and the blank control group（P 〈 0.05）.Compared with the negative group and the blank control group,the apoptosis rate in the transfection group was remarkably increased,while there was no significant difference in the negative group and the blank control group（P 〈 0.05）.Compared with negative group and blank control group,the expression of Caspase-9,Bax protein in the transfection group was remarkably increased,Bcl-2 was obviously decreased,there was no significant difference in the negative group and blank control group（P 〈 0.05）.Conclusion：Down-regulating the expression of GRP78 in the prostatic carcinoma cell can obviously inhibit the cell proliferation and promote cell apoptosis,and mitochondrial apoptotic pathway may involve the GRP78-mediated prostatic carcinoma cell proliferation.
作者
龚娟
卢童
GONG Juan;LU Tong(Department of Urology Surgery , The First People＇s Hospital of Tianmen, Tianmen 431700, China)
出处
《现代医学》
2018年第2期121-125,共5页
Modern Medical Journal
基金
湖北省自然科学基金资助项目(2016CFB523)
关键词
前列腺癌
葡萄糖调节蛋白78
线粒体凋亡通路
增殖
细胞凋亡
prostatic carcinoma
glucose-regulated protein 78
mitochondrial apoptotic pathway
proliferation
apoptosis
作者简介
龚娟（1977-），女，湖北天门人，主管护师，主要从事泌尿外科护理方向的研究。E—mail：gongjuan1977824@163．com.
