摘要
目的研究p65siRNA抑制NF-κB/p65信号通路对小鼠Lewis肺癌移植瘤细胞凋亡和Bax表达的影响。方法采用Lewis肺癌细胞C57BL/6雄性小鼠移植瘤组织悬液,建立C57BL/6雄性小鼠移植瘤模型。将NF-κB/p65 siRNA接种于荷瘤小鼠,观察下调NF-κB/p65表达后荷瘤小鼠瘤细胞凋亡情况。进一步应用实时定量PCR和Western blotting检测肿瘤组织中Bax mRNA及蛋白表达变化。结果经p65 siRNA治疗后,小鼠肿瘤细胞发生明显凋亡,肿瘤组织Bax m RNA及蛋白表达明显升高,差异均有统计学意义(P均<0.05)。结论 NF-κB/p65通路可能在Lewis肺癌细胞凋亡中发挥重要作用。
Objective To investigate the effects of nuclear factor-kappaB （NF-KB）/p65 signaling transduction on the apoptosis and expressions of apoptosis-related genes Bax in nude mouse lung tumour cell xenografts. Methods The nude mice Lewis lung carcinoma cell xenograft model was established, and then the mice were intraperitoneally injected with NF-κB/p65 small interfering RNA （siRNA）. The apoptosis of xenografted tumor cells in nude mice was detected by TUNEL assay. Expressions of Bax mRNA and protein were detected by RT-PCR and Western blotting. Results The result of TUNEL assay demonstrated that p65 siRNA evidently evoked cell apoptosis. Compared to the PBS treatment group or the normal control mice, both mRNA and protein expression levels of Bax in tumor xenografts were significantly up-regulated. There were significant differences among three groups （P 〈 0.05）. Condusion NF- κB/p65 subunit may play an essential role in cell apoptosis of Lewis lung tumor.
出处
《中国医科大学学报》
CAS
CSCD
北大核心
2017年第2期145-148,共4页
Journal of China Medical University
作者简介
曲耘（1968-），女，主治医师，博士．
通信作者：张晓晔，E-mail：Xiaoyezhangcn@163．com
