摘要
为建立一种用于检测胞内劳森菌的检测方法,本试验根据GenBank中公布的胞内劳森菌16SrRNA设计引物建立PCR诊断方法。结果显示:获得了大小为481bp的PCR产物,核苷酸序列同源性分析结果均为99%以上;特异性试验表明该方法对大肠杆菌、沙门菌、志贺菌、猪流行性腹泻病毒和金黄色葡萄球菌均为扩增阴性;敏感性试验表明该方法的最低检出量为32.8μg/L;对20份临床样品阳性检出率为15%。结果表明:该方法具有较好的特异性和敏感性,适用于临床检测胞内劳森菌。
To establish a PCR detection method of Lawsonia intracellularis, the primers were de- signed based on 16S rRNA which was published in Genbank. The 481 bp PCR products were gained. The analysis of nucleotide sequence homology were more than 99%. The specificity test were showed that the PCR method were negative to Escherichia coli, Salmonella, Shigella, Porcine epidemic diarrhea virus and Staphylococcus aureus. The lowest detection concentration of this method was 32.8 μg/L. The positive relevance ratio of 20 clinical samples was 15%. So this method had better specificity and sensibility, and could be used to detect Lawsonia intracellularis in the clinical test.
出处
《中国兽医学报》
CAS
CSCD
北大核心
2017年第1期36-39,共4页
Chinese Journal of Veterinary Science
基金
中国农业科学院创新工程基金资助项目(20140204066NY)
公益性行业(农业)科研专项经费项目(201303042)
关键词
胞内劳森菌
PCR
检测
Lawsonia intracellularis
PCR
detection
作者简介
孙娜（1983-），女，助理研究员，博士。
通讯作者，E—mail：tcscsp@126．com
