摘要
目的观察虫草益肾颗粒对高糖培养下人肾小球系膜细胞(HMC)的增殖及过氧化氢酶(CAT)、丙二醛(MDA)表达水平的影响,探讨其防治糖尿病肾病(DKD)的作用机制。方法将HMC随机分为6组:正常组、高糖组、福辛普利组、虫草益肾颗粒低、中、高剂量组,分别给予相应的含药血清处理后,用2-(2-甲氧基-4-硝苯基)-3-(4-硝苯基)-5-(2,4-二磺基苯)-2H-四唑单钠盐(CCK-8)检测24 h、48 h及72 h细胞增殖情况;收集48 h和72 h细胞上清液,用比色法分别检测各组CAT、MDA的表达水平。结果 HMC经高糖刺激后表现出明显增殖现象,且在24~48 h时增殖速度最快,48~72 h时增殖速度受抑制。各治疗组对高糖刺激下的HMC增殖均有抑制作用(P<0.01),且虫草益肾颗粒对HMC增殖的抑制作用呈时间与剂量依赖性关系。48 h、72 h时,与正常组比较,各组的CAT活性均明显下降(P<0.01),各组的MDA含量均明显上升(P<0.01),与高糖组比较,福辛普利组、虫草益肾颗粒低、中、高剂量组的CAT活性有明显上升趋势(P<0.05或P<0.01),MDA含量有明显下降的趋势(P<0.01)。结论虫草益肾颗粒益肾颗粒防治DKD的作用机制可能与促进自由基清除,抑制或阻断自由基引起的脂质过氧化反应,增强CAT活性,提高机体抗氧化能力有关。
Objective： To observe the effect of Chongcao Yishen Decoction on HMC proliferation and expression of CAT^MDA and to explore the mechanism of Chongcao Yishen Decoction in the treatment of DKD （Diabetic kidney disease）. Methods： HMC were divided into six groups ：the control group,the glucose group ,Fosinopril group,and the Chongcao Yishen Decoction low,medium and high dosage groups. Cells were collected after re- spectively given the appropriate treatment. The human glomerular mesangial cells proliferative degree within 24,48 and 72 hours were detected by CCK-8 colorimetry;cells supernatant in 48 and 72 hours was collected,and according to the specifications,the expression of CAT^MDA was detect with colorimetric method （DNS method）. Results： In vitro cultured glomerular mesangia] cells were obviously proliferated under the stimulation of high glucose. In the period from 24 h to 48 h ,the speed of proliferation was highest ,while in the period from 48 h to 72 h,the speed was inhibited. Each group had an inhibition on proliferation of HMC under the stimulation of high glucose （P〈 0.01 ）. Chongcao Yishen Decoction had a significant inhibition on proliferation of HMC ,and certain time and dose dependence has been detected. In 48 and 72 hours, compared with control group, CAT activity of other groups was significantly decreased （P〈 0.01 ）;the expression of MDA was significantly increased （P 〈 0.01 ）. Compared with the high glucose group, CAT activity in Fosinopril group and Chongcao Yishen Decoction low, medium and high dosage groups obviously tend to ascent（P〈 0.05 or P〈 0.01 ）;the expression of MDA had a significant lower tendency （P 〈 0.01 ）. Conclusion： The possible therapeutic mechanism of Chongcao Yishen Decoction for DKD is related to inhibiting lipid peroxidation,eliminating free radicals,raising CAT activities and iraproving the antioxidant capacity.
作者
宋立群
刘爽
宋业旭
贠捷
张睿
栾仲秋
SONG Liqun LIU Shuang SONG Yexu et al(Heilongjiang University of Traditional Chinese Medicine ,Heilongjiang,Harbin 150040, China.)
出处
《中国中医急症》
2016年第10期1833-1836,1854,共5页
Journal of Emergency in Traditional Chinese Medicine
基金
国家自然科学基金项目(81273911)
教育部高等学校博士学科重点专项科研基金资助项目(20112327110002)
黑龙江中医药大学研究生创新科研项目(yjscx2016005)
关键词
虫草益肾颗粒
肾小球系膜细胞
糖尿病肾病
氧化应激
Chongcao Yishen Decoction
Human glomerular memangial cell
Diabetic kidney disease
Oxidativestress
作者简介
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