摘要
对2个凡纳滨对虾（Litopenaeus vannamei）选育系（A和B）的自繁与杂交后代的生长性状及遗传多样性进行了分析.养殖对比实验结果表明,杂交组合AB生长性状优于其他各组,表现出较好的杂种优势.使用11对荧光标记的微卫星引物对4个凡纳滨对虾自繁与杂交群体以及1个从美国引进的初代亲本SIS群体的基因组DNA进行扩增,结果显示除AA群体外,其余4个群体均表现出较丰富的遗传多样性,观测到的平均等位基因数（Na）为4.273～5.636,平均期望杂合度（He）为0.586～0.629,平均多态信息含量（PIC）为0.512～0.556.遗传距离分析结果显示,SIS群体和AA群体的遗传距离最远（0.670 4）,而与AB群体的最近（0.131 4）.研究结果表明,引自美国的凡纳滨对虾群体经多代自繁后,其遗传多样性水平降低;而选育系间杂交则可使杂交后代的生长性状和遗传多样性水平得到改善.
The growth performance and genetic diversity of the inbred and crossbred progenies of two Litopenaeus vannamei selective lines（ A and B） were analyzed in this study.The results indicated that AB group showed the best growth performance and heterosis.Eleven fluorescently labeled microsatellite markers were used to amplify microsatellite DNA from five L. vannamei populations.The results showed that four populations（ except for AA） displayed higher genetic diversity with average numbers of alleles（ Na = 4. 273-5. 636）,average expected heterozygosity（ He = 0. 586-0. 629）,and average polymorphism information content（ PIC = 0. 512-0. 556）. Nei＇ s unbiased genetic distance（ Ds） indicated a close genetic relationship between populations SIS and AB（ Ds= 0. 131 4）,while populations SIS and AA showed the farthest genetic relationship（ Ds = 0. 670 4）.The results demonstrated that the genetic diversity decreased after several generations of selection,while hybridization between different selective lines could improve the growth performance and genetic diversity of L. vannamei.
出处
《厦门大学学报（自然科学版）》
CAS
CSCD
北大核心
2016年第5期646-653,共8页
Journal of Xiamen University：Natural Science
基金
厦门市海洋经济创新发展区域示范项目(13PZY001SF25)
关键词
凡纳滨对虾
生长性状
遗传多样性
微卫星
杂交
Litopenaeus vannamei
growth performance
genetic diversity
microsatellite
hybridization
作者简介
通信作者：wwyou@xmu．edu．cn
