摘要
[目的]利用离子交换层析方法分离与纯化口蹄疫病毒抗原。[方法]利用阴离子交换树脂制备高纯度的FMDV抗原,通过对样品及缓冲液pH、流速、缓冲液的盐浓度、样品上样体积等条件的摸索,确定最佳的FMDV抗原洗脱条件。[结果]当缓冲液pH为8.0、流速1.2 m L/min、盐浓度450 mmol/L、上样体积1.5 m L时,FMDV抗原的纯化效果最佳。[结论]该研究结果可为离子交换层析在FMDV抗原的分离与纯化提供依据。
[Objective] The aim was to separate and purify FMDV antigen by using ion exchange chromatography.[Method] High purity FM-DV antigen was prepared by anion exchange resin, through exploration on sample, buffer solution pH, current speed, salt concentration, load-ing volume of sample, the optimal elution condition of FMDV antigen was determined.[ Result] The best condition for purification of FMDV antigen is when buffer pH reached 8.0, flowed at a speed of about 1.2 mL/min, salt concentration was 450 mmol/L and the loading volume of sample was 1.5 mL.[Conclusion] The results can provide basis for separation and purification of FMDV antigen by ion exchange chroma-tography.
出处
《安徽农业科学》
CAS
2016年第22期138-139,共2页
Journal of Anhui Agricultural Sciences
关键词
阴离子交换树脂
口蹄疫病毒抗原
纯化
Anion exchange resin
Foot and mouth disease virus antigen
Purification
作者简介
马全英（1981-），女，甘肃兰州人，助理研究员，硕士，从事分子免疫学方面的研究。
