摘要
利用ATP适体修饰磁珠为分离载体,以电化学试剂结晶紫(CV)适体、CV、ATP适体互补序列修饰的胶体金CV/DNA2/DNA3/AuNPs为探针,建立了测定ATP电化学新方法。方法检测限为1.0×10^(-9) mol·L^(-1),另外,该方法对ATP的测定表现出了良好的选择性。并利用该方法对运动前后小鼠心肌ATP含量进行了测定。
ATP aptamer was immobilized onto the surface of magnetic beads. Then the probe which was labeled with aptamer complementary sequence and crystal violet aptam- er was hybridizated onto it. The introduction of ATP induced release of the probe. The electrochemical signal was produced from the released probe. The experimental results demonstrated it can be to detect 1.0×10^- 9mol · L^-1 ATP. The method also showed excellent selectivity toward GTP,UTP 和 CTP. The protocol was successfully applied to the determination of ATP in cardiac musculus.
出处
《青岛科技大学学报（自然科学版）》
CAS
2016年第3期245-248,269,共5页
Journal of Qingdao University of Science and Technology:Natural Science Edition
基金
国家自然科学基金项目(21275085
21575073)
作者简介
宗迎夏（1990-），女，硕士研究生．
