摘要
目的：测定维吾尔药腺果蔷薇根、茎、叶和果实中总黄酮含量。方法：采用单因素与正交实验确定最佳提取工艺；采用亚硝酸钠-硝酸铝-氢氧化钠比色法测定腺果蔷薇不同部位的总黄酮含量。结果：最佳提取工艺为：乙醇浓度为60％，用量为药物重量的14倍，加热回流2h，2次。以芦丁为对照品求得线性方程为：Y=0．5281X-0．0149（r=0．9998），线性范围为0．2-1．2mg/mL。结论：该提取工艺稳定可行，含量测定方法简便、灵敏、准确，可作为腺果蔷薇中总黄酮的含量测定方法。
Objective： To determine the content of the total flavones from the roots, stems, leaves and fruits of Rosa fedtschenkoana Regel. Methods： The best extraction technology was determined by orthogonal experiment design; The content of total flavones was determined by absorption spectrometry with rutosids as reference sub- stance. Results： Optimum extraction technology was as follows, 60% ethanol, ratio of material to liquid 14 times, and time of 2 h, twice. The standard to make the standard curve： y=0.5281X-0.0149（r=0.9998）; the linear range was 0.2-1.2 mg/mL. Conclusion： This method was simple, accurate and suitable for the quality control of the flavonoids in Rosa fedtschenkoana Regel.
出处
《中医药导报》
2016年第11期36-39,共4页
Guiding Journal of Traditional Chinese Medicine and Pharmacy
基金
国家自然科学基金资助项目(No.81360668)
关键词
腺果蔷薇
总黄酮
提取工艺
含量测定
Rosa fedtschenkoana Regel.
total flavones
extraction technology
contents determination
作者简介
通讯作者：杨梅英，E-mail：115149469@qq．com
