摘要
选用6份有代表性的陕西茶树资源材料,从121条SCoT引物中筛选出38条扩增条带清晰、多态性高的引物,对陕西省50个茶树资源进行遗传多样性研究.结果表明,SCoT标记能够揭示材料间较高的遗传多样性,每条引物可检测到4-17个等位位点,平均为11个,38条SCoT引物共扩增出414条DNA片段,其中400条具有多态性,多态性比率（PPB）为96.62%.每个SCoT位点的遗传多态性信息含量在0.56-0.99之间,平均为0.90.供试材料的遗传相似系数集中在0.59-0.71之间.研究表明,SCoT标记可以用来对茶树进行遗传基础研究,基于SCoT标记的陕西省主要茶树资源遗传多样性较高.
Genetic diversity of 50 tea germplasm materials in Shaanxi was investigated. Taking 6 representative Shaanxi tea germplasm materials as templates, 38 of 121 SCoT marker primers were screened out, which showed specific bands and highly polymorphism. The results showed that SCoT markers could reveal high genetic diversity among tea germplasm, and each primer can detect out 4 to 17 alleles. On average, 11 allelic variations could be detected by each SCoT marker. 414 DNA fragments were amplified via 38 pairs of SCoT primer, of which 400 showed polymorphism and the ratio (PPB) was 96.62%. In addition, genetic polymorphism information content of each SCoT locus was between 0.56 and 0.99, the average of which was 0.90 for the tested materials, and the genetic similarity coefficient was between 0.59 and 0.71. The research showed that SCoT marker could be used in the genetic diversity research of tea germplasm. Basing on SCoT marker, genetic diversity level of these Shaanxi tea germplasms were relatively high in the genetic diversity.
出处
《茶叶科学》
CAS
CSCD
北大核心
2016年第2期131-138,共8页
Journal of Tea Science
基金
陕西省自然科学基础研究计划项目(2013JZ008)
陕西省科技统筹创新工程(2013KTZB02-01-01)项目
关键词
茶树
SCoT
多样性
tea(Camellia sinensis)
SCoT
diversity
作者简介
陈熙,女,硕士研究生,主要从事植物分子生物学研究.
zy68169@sina.com
