摘要
‘云烟87’和‘K326’花期喷施多胺及其合成抑制剂，收集其花粉测定多胺含量和花粉活力。研究结果表明：喷施外源多胺可不同程度提高花粉内源多胺含量、保护酶活性和花粉萌发率，多胺合成抑制剂则降低花粉内源多胺含量、保护酶活性和花粉萌发率。其中，喷施0．5mmol／L腐胺（Put）效果最好，花粉内源多胺含量和保护酶活性较高，花粉萌发率提高10．03％～34．00％。研究结果说明：烟草花期喷施适宜浓度的多胺可以提高花粉多胺含量和花粉活力，可以作为一种调控花粉活力的方法。
Pollens from ＇Yunyan 87＇ and ＇K326＇ were collected after spraying polyamines and polyamine synthesis inhibitor at flowering stages. Polyamines content and vigor of pollen were tested. The results showed that spraying exogenous polyamines improved polyamines content, protective enzyme activity and germination rate of pollen in different extent. Polyamine synthesis inhibitors had negative effect on polyamines content, protective enzyme activity and germination rate of pollens. The effect of 0.5 mmol/L putrescine was better than other treatments, polyamines content and protective enzyme activity of pollen were higher, the germination rates of pollen were increased by 10.03% to 34.00%. The results indicated that spraying suitable concentration of polyamines at flowering stages could improve polyamines content and vigor of pollen, which was used a method of regulation of pollen vigor.
出处
《云南农业大学学报（自然科学版）》
CSCD
北大核心
2016年第1期122-126,共5页
Journal of Yunnan Agricultural University:Natural Science
基金
中国烟草总公司(110201302006)
云南省烟草公司资助项目(2011YN09
2013YN10)
关键词
多胺
多胺合成抑制剂
花粉活力
polyamine
polyamine synthesis inhibitor
pollen vigor
作者简介
索文龙（1980-），男，山西长治人，硕士研究生，主要从事烟草种子科学方面研究。E-mail：suowenlong@163．com。
通信作者Corresponding author：马文广（1972-），男，云南玉溪人，硕士，研究员，主要从事烟草育种与种子科学方面研究。E-mail：mwg@tobacco-seed．com
