摘要
目的探讨胃癌组织中高迁移率族蛋白A2(HMGA2)及基质金属蛋白酶-9(MMP-9)表达与肿瘤侵袭和转移能力的关系及预后意义。方法采用免疫组化法(SP法)检测93例胃癌组织、30例正常胃黏膜组织中HMGA2、MMP-9的表达;收集患者临床病历资料,并进行随访。结果 HGMA2蛋白在胃癌组织中阳性表达率分别为明显高于正常胃黏膜(P<0.01);MMP-9蛋白在胃癌组织中阳性表达率明显高于正常胃黏膜(P<0.01)。HMGA2与MMP-9在胃癌组织中的蛋白阳性表达均与胃癌组织的肿瘤浸润深度、肿瘤分化程度、淋巴结转移、TNM分期有关(P<0.05);与患者的性别、年龄、肿瘤直径无关(P>0.05)。相关性分析发现,HMGA2与MMP-9在胃癌组织中的表达情况呈正相关(r=0.317,P<0.01)。经Kaplan-Meier生存分析显示,HMGA2、MMP-9蛋白表达阳性患者的生存率均低于表达阴性患者(P<0.01)。结论 HMGA2、MMP-9与胃癌浸润和转移有关,两者表达具有相互协同作用,对胃癌的侵袭和转移起重要的促进作用。HMGA2、MMP-9是影响预后的危险因素,可能成为胃癌治疗的新靶点。
Objective To investigate the expression of high mobility group protein A 2 （ HMGA 2 ） , matrix metalloproteinase-9 （MMP-9） and their relationship with the invasion and metastasis of gastric cancer ,and to explore their significance in patients’ prognosis.Methods Immunohistochemistry was used to examine the expression of HMGA 2,MMP-9 proteins in gastric carcinoma （ n =93） and normal gastric mucosa tissue （ n =30）.Furthermore the clinical case history data were colleted and follow-up was carried out.Results The positive expression rates of HMGA2 （73.1%） and MMP-9 expressions （80.6%） in gastric cancer tissues were significantly higher than those in normal gastric tissues （13.3% and 23.3%,respectively, P ＆lt;0.01）.The positive expressions of HMGA2 and MMP-9 in gastric cancer tissues were closely correlated to the infiltration degree , differentiation degree , lymphatic metastasis and TNM staging of gastric cancer （ P ＆lt;0.05）,however,whcih were not correlated to patients ’ sex,age and tumor’ s diameter （ P ＆gt;0.05）.The expression of HMGA2 was positively related with that of MMP-9 in gastric cancer tissue （ r =0.317, P ＆lt;0.01）.The survival rates in patients with positive expression of HMGA2,MMP-9 were significantly lower than those in patients with negative expression （ P＆lt;0.01）.Conclusion HMGA2 and MMP-9 are correlated with infiltration and metastasis of gastric cancer ,there is a synergistic reaction in the expression of two factors ,which play an important role in promoting invasion and metastasis of gastric cancer . HMGA2 and MMP-9 are the risk factors of affecting prognosis ,which may become new target in treatment of gastric cancer .
出处
《河北医药》
CAS
2014年第6期819-822,共4页
Hebei Medical Journal
