摘要
目的观察Apelin-13对实验性自身免疫性神经炎(EAN)大鼠淋巴结中炎症因子肿瘤坏死因子-α(TNF-α)、白细胞介素-6(IL-6)和干扰素-γ(IFN-γ)表达的影响。方法采用周围神经髓鞘抗原(P257-81)注射入Lewis大鼠后肢足垫诱导EAN模型。Lewis雄性大鼠随机分为对照组、EAN模型组和Apelin处理组。Apelin处理组于免疫当天至第15天每天尾静脉注射Apelin-13(0.1 mg/kg)。观察各组大鼠发病情况和坐骨神经组织病理学变化,采用实时定量PCR和Western blot检测淋巴结中TNF-α、IL-6和IFN-γ表达。结果与EAN模型组比较,Apelin处理组大鼠最初发病时间明显延长,高峰期临床评分显著降低,炎症因子TNF-α、IL-6和IFN-γ的表达显著降低(均P<0.05)。结论 Apelin-13对实验性自身免疫性神经炎大鼠有治疗作用,其机制可能与下调TNF-α、IL-6和IFN-γ的表达有关。
Objective To observe the effects of Apelin-13 on the expression of tumor necrosis factor-α （TNF-α）,interleukin-6 （IL-6） and interferon-γ （IFN-γ） of lymph node in experimental autoimmune neuritis （EAN） rats.Methods The rats models of EAN were established by injection of peripheral nerve myelin sheath antigen （P257-81） in the foot pad of male Lewis rats.The rats were randomly divided into the control group,EAN model group,and Apelin treatment group.The rats in Apelin treatment group were injected with Apelin-13 （0.1 mg/kg） by tail vein once daily from the 1st day to the 15th day.The clinical incidence and pathology in the rats were assessed.The expressions of TNF-α,IL-6 and IFN-γin lymph node were measured by real-time PCR and Western blot respectively.Results Compared with the EAN model group,the initial time of nervous symptom significantly increased,the maximal neurological score significantly decreased,the expressions of TNF-α,IL-6 and IFN-γ in lymph node significantly decreased in Apelin treatment group （all P ＜ 0.05）.Conclusion Apelin-13 can treat the EAN in EAN rats model,the mechanism of which may be related with down-regulation of TNF-oα,IL-6 and IFN-γ.
出处
《中国医药导报》
CAS
2014年第4期17-20,共4页
China Medical Herald
作者简介
吴峥嵘（1980.6-）,女,硕士,主要从事神经系统免疫性疾病及变性疾病防治研究.
【通讯作者】谭利明，男，博士，教授，博士生导师。
