摘要
目的:观察针灸预处理"内关"穴对缺血再灌注损伤兔心肌线粒体内缝隙连接蛋白(Cx43)表达的影响,并比较电针与艾灸的作用差异。方法:将32只新西兰大耳白兔随机分为4组,每组8只,即模型组、电针内关组、艾灸内关组、假手术组。前3组建立兔心肌缺血再灌注损伤模型,电针内关组与艾灸内关组分别于造模成功前后予以电针、艾灸"内关"穴20min,实验结束后在光学显微镜下观测Cx43的分布情况,并检测心肌细胞中Cx43光密度(IOD)均值。结果:在光学显微镜下模型组Cx43分布明显散乱、稀疏,而其他3组可见较多Cx43表达,呈条状、链状、不规则状,与细胞长轴垂直,部分形成侧侧连接与长轴平行,且艾灸内关组Cx43表达的形态及分布特征与电针内关组无明显差异。心肌细胞Cx43表达的IOD均值,电针内关组(735.10±152.01)、艾灸内关组(836.15±247.10)及假手术组(950.56±223.37)均高于模型组(312.68±105.20),且电针内关组与艾灸内关组中心肌细胞Cx43表达的IOD均值差异无统计学意义。结论:艾灸、电针预处理兔"内关"穴均可使心肌细胞Cx43表达升高,参与心肌电耦联和代谢耦联,来保护缺血再灌注的心肌细胞。且电针和艾灸"内关"穴对心肌细胞Cx43表达均值无显著差异。
Objective To observe change of expression level of Connexin 43 （Cx 43） in myocardial mitochondria of ischemia reperfusion injury after pretreatment of acupuncture and moxibustion at ＂Neiguan＂ （PC 6） in rabbits, and compare the different effect between electroacupuncture and moxibustion. Methods Thirty-two New Zealand big-eared white rabbits were randomly divided into a model group, an electroacupuncture group, a moxibustion group and a sham-operation group, 8 cases in each one. The model of myocardial ischemia reperfusion injury was established in the first three groups. Before and after animal model was successfully made, the electroacupuncture and moxibustion were applied at ＂Neiguan＂ （PC 6） for 20 min respectively in the electroacupuncture group and moxibustion group. After the experiment, distribution of Cx 43 was observed under optical microscope while mean value of integral optical density （IOD） of Cx 43 in myocardial cell was tested. Results The distribution of Cx 43 in the model group was obviously scattered and sparse, but more expression of Cx 43 could be seen in the other three groups that were shaped as strip, chain or irregular and was perpendicular to cell long axis or formed side-side con- nection that was parallel to long axis. Shape and distribution of Cx 43 expression in the moxibustion group were not obviously different from that in the electroacupuncture group. The mean value of IOD of Cx 43 expression in myocardial cell in the electroacupuncture group （735. 10 ±152.01）, moxibustion group （836. 15 ±247. 10） and sham-operation group （950. 56±223.37） was higher than that in the model group （312.68±105.20）, and differe- nce of the mean value in the electroacupuncture group was not statistically significant from that in the moxibustion group. Conclusion The pretreatment of acupuncture and moxibustion at ＂Neiguan＂ （PC 6） in rabbit could increase expression of Cx 43 in myocardial cell and participate in electrical coupling and metabolic coupling to protect myocardial cell from ischemia reperfusion. However, differences of mean value of Cx 43 expression in myocardial cell were not statistically significant during electroacupuncture and moxibustion at ＂Neiguan＂ （PC 6）.
出处
《中国针灸》
CAS
CSCD
北大核心
2013年第11期1013-1016,共4页
Chinese Acupuncture & Moxibustion
基金
国家自然科学基金重点项目:81072868
湖南省教育厅课题项目:08C633
关键词
心肌缺血再灌注损伤
预处理
艾灸法
电针
内关
CX43
ischemia-reperfusion injury
pretreatment
moxibustion
electmacupuncture
PC 6（Neiguan）
connexin 43
作者简介
通信作者：高智颖（1973-），女，副主任医师。研究方向：针灸治病机制的研究。E—mail：84038566@qq．com
