摘要
目的 :研究氯化钐 ( Sm Cl3 )、氯化镨 ( Pr Cl3 )对地塞米松 ( DX)所致免疫功能低下小鼠腹腔巨噬细胞 ( PM)的影响。方法 :选用昆明小鼠连续肌注 DX建立免疫功能低下模型后 ,腹腔注射0 .0 5mg/kg,0 .5mg/kg Sm Cl3 和 Pr Cl3 ,连续 7d,采用定量细胞化学、电子显微镜及吞噬实验方法观察 PM的形态结构及功能。结果 :两种剂量 Sm Cl3 和 Pr Cl3 对 DX所致免疫功能低下小鼠 PM,可使酸性磷酸酶 ( ACP)、α-醋酸萘酚酯酶 ( ANAE)含量和刀豆蛋白 A受体 ( Con AR)数目明显增多。电镜下 ,PM胞质内溶酶体增多 ,表面皱褶及微绒毛增多。吞噬中性红染料的能力增强。结论 :Sm Cl3和 Pr Cl3 对免疫功能低下小鼠
Objective To investigate the influence of Samarium chloride (SmCl 3) and Praseodymium chloride (PrCl 3) on peritoneal macrophage (PM) in mice of low immune function due to Dexamethasone (DX).Methods The mice of Kunming species were selected as experimental animals.They were given DX i.m, in order to set model of low immune function and given 0.05 mg/kg and 0.5 mg/kg of SmCl 3 and PrCl 3 i.p for 7 days.The structure and function of PM were observed with quantitative cytochemistry electron microscope and phagocytic test.Results The two dosages of SmCl 3 and PrCl 3 not only increased the contents of ACP,ANAE and the numbers of ConA receptors in PM of low immune function mice,but also improved the phagocytosis of PM in neutral red.Under electron microscope,the lysosomes were increased on plasma of PM,and folds and microvilli on surface of PM were raised.Conclusion The SmCl 3 and PrCl 3 had stimulation in structure and function of PM in mice of low immune function.
出处
《白求恩医科大学学报》
CSCD
2000年第1期29-31,共3页
Journal of Norman Bethune University of Medical Science
关键词
氯化钐
氯化镨
免疫功能低下
腹腔
巨噬细胞
samarium chloride
praseodymium chloride
peritoneal macrophage
quantitative cytochemistry
low immune function
electron microscope
