摘要
研究了花期施用PCPA和2,4-D对发育过程中番茄果实糖含量、蔗糖代谢相关酶和果糖激酶活性及果糖激酶基因表达的影响。结果表明,随着番茄果实的发育,果糖激酶的活性及其基因表达均先上升后下降,成熟时达到最低,但FRK1基因表达量较高;果糖和葡萄糖含量呈递增的趋势,成熟时含量达到最高。PCPA和2,4-D处理后降低了成熟番茄果实果糖激酶活性,提高了酸性转化酶活性,促进了FRK1基因表达,提高了成熟番茄果实中果糖和葡萄糖的含量。
Sugar contents,enzyme activities related to sucrose metabolism and fructokinase（FRK）,fructokinase gene expression were studied during tomato fruit development after PCPA and 2,4-D treatment,respectively.Results showed that activity and gene expression of fructokinase increased at first then decreased during fruit development in control.Fructokinase activity reached the lowest level,but FRK1 gene expression showed a higher level during fruit ripening stage.However,the contents of fructose and glucose in fruit reached the peak value at the maturity stage.The fructokinase activity declined,while the activity of acid invertase was increased went up.At the same time gene expression of fructokinase was enhanced during fruit maturing stage after treated with PCPA and 2,4-D,which made the contents of fructose and glucose increased.
出处
《核农学报》
CAS
CSCD
北大核心
2011年第5期1047-1051,共5页
Journal of Nuclear Agricultural Sciences
基金
国家十二五科技支撑项目(2011BAD12B03)
辽宁省教育厅科学技术研究项目(2008623)
关键词
番茄
生长调节剂
果糖激酶
基因表达
Solanum lycopersicum
growth regulator
fructokinase
gene expression
作者简介
史宝中（1968-），男，辽宁盘锦人，硕士，副研究员，主要从事设施园艺的研究及实验室建设。Tel：024—86574424；E-mail：synua@163．com
通讯作者：崔娜（1968-），女，辽宁桓仁人，博士，副教授，主要从事设施园艺及发育生物学的研究。Tel：024-88487163；E-mail：syaua@163．com
