摘要
不同因素及水平正交试验结果表明,使用ABT6400 mg/L+IBA200 mg/L溶液浸泡山苍子(Litsea cube-ba)继代芽15 min,扦插于红心土基质中培养,是较好的组合,生根率达92.1%。除7、8、9月份外,其它时间均能进行山苍子继代芽瓶外生根,生根率80%以上,其中5月份最高,达92.3%。山苍子组培继代芽瓶外扦插初期适宜置于封闭式的50 cm高塑料拱棚内进行管护。
The non-tube-rooting technique by using subcultured bud of Litsea cubeba was studied. The result of orthogonal test with different factors and levels demonstrated that it was the best method to immerse the subcultured buds for 15 min in ABT6400 mg/L + IBA200 mg/L solution and cultivate in subsoil. By using this method the rooting rate reached 92.1%. Excluding July, August and September, the rooting rate exceeded 80 % in other months, and the highest rate reached 92.3 % in May. In the preliminary stage of rooting it was preferable to be protected in closed plastic shed at the height of 50cm.
出处
《广西林业科学》
2011年第3期169-172,共4页
Guangxi Forestry Science
基金
广西林业科技项目(桂林科字〔2006〕第24号)
关键词
山苍子
继代芽
瓶外生根
Litsea cubeba
subcultured bud
non-tube-rooting
作者简介
吴幼媚(1959-),女,教授级高工。主要从事林木组织培养研究。