摘要
为了提高蕨菜孢子人工培养成苗效率，对４ 种培养基质和赤霉素（ＧＡ３）、硼３ 种使用水平的孢子萌发成苗效果进行了研究。结果表明，森林土、琼脂和蛭石间原叶体数无差异，但成苗率间差异显著；森林土成苗率最高，达到４２．８６％ ；砂基上孢子虽能萌发产生片状体，但不能发育到性器官形成期；赤霉素对孢子萌发生长具有显著的促进作用，在含有０．１５×１０－ ７ ｇ／Ｌ赤霉素培养基上成苗率较高；硼可提高配子体受精成苗，并以０．０５％ 硼酸处理效果最好；赤霉素与硼有显著的互作效应，含有０．１５×１０－ ７ ｇ／Ｌ赤霉素培养基上生长的原叶体，在性器官形成期喷施０．０５％ 硼酸，成苗率高达５１．６０％ 。
In order to raise efficiency of planting seedling by artificial spore culture of Pteridium aquilinum, the effects of 4 cultural ground substances and 3 levels of GA 3 and B applying on spores germination and planting rate were studied. The results showed that there was no significant difference of the numbers of prothallus induced from spores by forest soil, agar and vermiculite, but the planting rates among them were significantly different. The planting rate of prothallus on the forest soil was the maximum in selected ground substances, about 42.86%. Although spores could germinate and generate young prothallus on the sand base, they could not grow to the period of formation of reproductive organ. GA 3 had obvious improving roles on spore germination and growth. The planting rate of prothallus was fairly high at 0.15×10 -7 g/L GA 3 treatment. B can promote gametophyte fertilizing and formation of planting seedling. The optimum density of boron acid application was 0.05%. The interaction GA 3×B had been highly significant.In the forming stage of reproductive organ, spraying 0.05% boron acid on the prothallus, cultured in the base added 0.15×10 -7 g/L GA 3, makes planting rate to reach 51.61%.
出处
《草业学报》
CSCD
1999年第1期62-68,共7页
Acta Prataculturae Sinica
基金
甘肃省自然科学基金
关键词
蕨菜
孢子
人工培养
培养基质
赤霉素
硼
成苗
Pteridium aquilinum, spores, artificial culture, cultural ground substances, GA 3, boron(B)
