摘要
目的表达B病毒糖蛋白，筛选出具有较好检测敏感性和特异性的抗原或抗原组合，用以检测猴血清中的抗体。方法原核表达B病毒糖蛋白C，D和G，经纯化、复性后用蛋白印记验证其抗原性，建立ELISA法，对方法进行评价。结果3个表达抗原反应特异性较好；以BV-ELISA为标准，各抗原的检测敏感性分别是：BVgC-ELISA84.6％，BVgD-ELISA88.5％，BVgC＋gD-ELISA96.2％，检测特异性分别是：BVgC-ELISA93％，BVgD-ELISA85.7％，BVgC＋gD-ELISA64.3％。结论表达的3个BV重组抗原都具备作为替代抗原检测BV抗体的潜力。
Objective To express the glycoproteins of simian B virus, use them to detect the BV antibodies in serum,and search for the better antigens or their combination which have best sensitivity and specificity. Methods Express B virus glycoprotein C,D and G and check their antigenicity by Western Blot after purification and renaturation.The ELISA was established and evaluated. Results The three antigens react well with simian serum which BV antibody is positive.The sensitivity of the antigens is BVgC-ELISA 84.6%, BVgD-ELISA 88.5%, BVgC＋gD-ELISA 96.2%, specificity is BVgC-ELISA 93%, BVgD-ELISA 85.7%, BVgC＋gD-ELISA 64.3% according to the results of BV-ELISA. Conclusion The three antigens react well with simian serum and can be used as surrogate antigens of BV.
出处
《实验动物与比较医学》
CAS
2009年第3期162-167,176,共7页
Laboratory Animal and Comparative Medicine
基金
北京市科委实验动物法制管理与科技支撑平台（课题编号：Z0004100040691-1）.
作者简介
李晓波（1980-），男，免疫学硕士。研究方向：实验动物病毒学。E-mail：lxb8493059@yahoo．com．cn
通讯作者：贺争鸣（1957-），男，博士，研究员，硕士生导师。研究方向：实验动物病毒学。E-mail：zhengminghe57@163．com
