摘要
目的建立高效液相色谱法测定双黄连注射液中黄芩苷外标定量的方法。方法采用RP—HPLC法，色谱柱为Kromasil C18（150×4．6mm，5μm）；流动相：甲醇-1％冰醋酸（50：50），流速为1ml／min，紫外检测波长为280nm。结果黄芩苷的线性范围为3．772～94．30μg／ml；相关系数为0．9993；加样回收率为95．74％；检测限为25．7ng；精密度实验RSD为1．7％；重现性实验RSD为1．94％；稳定性实验RSD为1．87％；三批样品中黄芩苷的含量为11．94mg／ml，13．47mg／ml，16．43mg／ml。结论该方法简便、快速、准确、灵敏度高、重现性好，可用于该制剂的含量测定。
Objective： To develop a HPLC method for the determination of baicalin in shuanghuanglian inoculation fluid. Method： The method used the RP - HPLC , the chromatographic column was Kromasil C18 （ 150 ×4.6mm, 5μm） , the mobile phase was methyl alcohol- 1% glacial acetic acid （50： 50）, the speed of flow was 1ml/min, the ultraviolet examination wave length was 280nm. Results： The baicalin linearity range is 3. 772 - 94.30μg/ml, the correlation coefficient was 0. 9993 ; the average recoveries of adding sample is 95.74% , the examination limits is 25.7ng , the accuracy tests RSD （ n = 5） is 1.7% , the reproduction quality tests RSD （ n = 5） is 1.94% , the stability tests RSD （ n = 5） is 1.87%. In three batch of samples the baicalin content are 11.94mg/ml, 13.47mg,/ml, 16.43mg/ml. Conclusion： The method is simple, fast, accurate, the sensitivity is high, the reproduction quality is good, and it may be used in the the content determination.
出处
《湖北中医学院学报》
2008年第3期53-54,共2页
Journal of Hubei College of Traditional Chinese Medicine
作者简介
杨瑞芬（1956-），女，湖北中医学院副教授。
通讯作者：彭家钢，男，湖北中医学院副教授。
