摘要
目的初步研究转录因子Pax6(paired homebox 6)在大鼠触须毛囊中的表达。方法运用免疫组织化学法检测Pax6蛋白在大鼠触须毛囊及培养的毛囊bulge细胞中的表达,RT-PCR分析毛囊中Pax6两种亚型的表达情况。结果大鼠触须毛囊细胞中Pax6表达阳性,RT-PCR显示表达量以Pax6-5a为高,在体外培养细胞中表现出细胞增殖能力与Pax6在细胞中的表达部位相关。结论Pax6在大鼠触须毛囊中表达,可能在其形成和功能的维持上发挥一定的作用。
Objective To investigate the expression pattern of transcription factor, paired homebox 6 （Pax6） in rat whisker hair follicle. Methods Whisker hair follicle was collected from 5 7-day-old SD rats. Immunohistochemical assay was carried out to detect Pax6 in the follicle. Pax6 mRNA expressions and its splicosome, Pax6-5a were detected by RT-PCR. Whisker hair follicle was primarily cultured to harvest rat hair fol- licle bulge cells. Then expressions of Pax6, CD34, α6-integrin, and Ki67 were detected in the obtained cells by immunofluorescent method. Results Pax6 was positively expressed in rat whisker hair follicle. The mRNA expression of Pax6-5a was stronger than that of Pax6. The primarily cultured cells expressed CD34 and α6-integrin in the membrane. Pax6 was mainly located in the nuclei of the cells with Ki67, and little in the cytoplasm. The proliferative ability of the cells was correlated with the location of Pax6 protein. Conclusion Pax6 expresses in rat whisker hair follicle. Its expression patterns indicate that it may be involved in the formation and function of whisker hair follicle.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
2008年第13期1296-1298,共3页
Journal of Third Military Medical University
基金
重庆市科委自然科学基金(2003BB5007)~~
关键词
PAX6
毛囊
paired homebox 6
hair follicle
作者简介
杨珂，女，陕西省潼关市人，博士研究生，助教，主要从事上皮生物学方面的研究。电话：（023）68752259，E-mail：78210114@sohu．com
通信作者：杨恬，电话：（023）68752260
