摘要
目的:探讨树突状细胞(DCs)活化的细胞毒T细胞(CTLs)联合5-氟尿嘧啶(5-FU)在裸鼠体内外对子宫颈癌的抑制作用。方法:MTT法检测DCs激活的CTLs联合5-FU在裸鼠体外对子宫颈癌细胞的抑制作用。裸鼠体内实验分为CTLs治疗组、5-FU治疗组、CTLs/5-FU治疗组及对照组,观察移植瘤成瘤率、潜伏期、瘤重及瘤体积。结果:治疗组在裸鼠体外都显著抑制子宫颈癌细胞生长,CTLs/5-FU治疗组最显著(P<0.01)。裸鼠体内实验中,治疗组的成瘤率、瘤体积、瘤重都显著降低,潜伏期延长,CTLs/5-FU治疗组最显著(P<0.01)。结论:联合应用DCs激活的CTLs及5-FU比单独应用CTLs或5-FU更有效。
Objective： To investigate the inhibitive effect of cytotoxic T lymphocytes （CTLs）, actived by dendritic cells （DCs）, associated with 5 -fluorouracil （5 - FU） on carcinoma cervicis in vitro and in nude mice. Methods： The inhibitive effect of CTLs, actived by DCs, associated with 5 - FU on carcinoma cervicis was detested in vitro by MTr methods. Additionally, there were four groups in experiments of nude mice ： control, CTLs, 5 - FU and CTLs/5 - FU groups. These treatements given before or after the mice were attacked by carcinoma cervicis cells and the tumor generating rate, the latent phase, the volume and weight of tumor were observed. Results： The inhibitive effect of three therapy groups was significantly increased, especially the CTLs/5 - FU group in vitro （ P 〈 0.01 ） . Additionally, in vivo, the latent phase of therapy groups increased and the tumor generating rate, the volume and weight of tumor decreased too, especially the CTLs/5 - FU group （P 〈0. 01） . Conclusion： CTLs, actived by DCs, associated with 5 - FU are more effective to carcinoma cervicis than CTLs or 5 - FU.
出处
《中国妇幼保健》
CAS
北大核心
2008年第16期2287-2288,共2页
Maternal and Child Health Care of China
基金
辽宁省自然基金资助项目(001082)
作者简介
通讯作者
