摘要
AIM: To investigate the effects of exogenous melatonin on bacterial translocation and apoptosis in a rat ulcerati-ve colitis model. METHODS: Rats were randomly assigned to three groups: groupⅠ: control, group Ⅱ: experimental colitis, group Ⅲ: colitis plus melatonin treatment. On d 11 after colitis, plasma tumor necrosis factor-α, portal blood endotoxin levels, colon tissue myeloperoxidase and caspase-3 activity were measured. Bacterial translocation was quantified by blood, lymph node, liver and spleen culture. RESULTS: We observed a significantly reduced inciden-ce of bacterial translocation to the liver, spleen, mesen-teric lymph nodes, portal and systemic blood in animals treated with melatonin. Treatment with melatonin signifi-cantly decreased the caspase-3 activity in colonic tissues compared to that in trinitrobenzene sulphonic acid-trea-ted rats (16.11 ± 2.46 vs 32.97 ± 3.91, P < 0.01). CONCLUSION: Melatonin has a protective effect on ba-cterial translocation and apoptosis.
AIM： To investigate the effects of exogenous melatonin on bacterial translocation and apoptosis in a rat ulcerati-ve colitis model. METHODS： Rats were randomly assigned to three groups： groupⅠ： control, group Ⅱ： experimental colitis, group Ⅲ： colitis plus melatonin treatment. On d 11 after colitis, plasma tumor necrosis factor-α, portal blood endotoxin levels, colon tissue myeloperoxidase and caspase-3 activity were measured. Bacterial translocation was quantified by blood, lymph node, liver and spleen culture. RESULTS： We observed a significantly reduced inciden-ce of bacterial translocation to the liver, spleen, mesen-teric lymph nodes, portal and systemic blood in animals treated with melatonin. Treatment with melatonin signifi-cantly decreased the caspase-3 activity in colonic tissues compared to that in trinitrobenzene sulphonic acid-trea-ted rats （16.11 ± 2.46 vs 32.97 ± 3.91, P 〈 0.01）. CONCLUSION： Melatonin has a protective effect on ba-cterial translocation and apoptosis.
作者简介
Correspondence to： Alper Akcan, MD, Department of General Surgery, Erciyes University School of Medicine, Kayseri 38039, Turkey. acakcan2002@yahoo.com Telephone： ＋90-533-7430357 Fax： ＋90-352-4375273
