摘要
目的观察survivin在塞来昔布诱导非小细胞肺癌(NSCLC)细胞株凋亡中的作用。方法用MTT法检测细胞株的增殖,用流式细胞学、透射电镜检测细胞株的凋亡,用RT-PCR、W estern b lot检测survivin的mRNA及蛋白表达。结果①塞来昔布对NSCLC有明显的剂量依赖性和时间依赖性抑制作用,塞来昔布对鳞癌细胞株NC I-H520的抑制率明显高于对腺癌细胞株A549的抑制率;②塞来昔布诱导NSCLC凋亡,凋亡随剂量的增加而增加;③塞来昔布作用后survivin的mRNA及蛋白表达减少。结论塞来昔布可能是通过降低survivin的mRNA及蛋白表达水平,诱导NSCLC细胞凋亡。
Objective To investigate the inhibitory effect of celecoxib on non-small cell lung cancer in vitro and the role of survivin. Methods The inhibitory effect of celecoxib was detected by MTI＇. Flow cytometry and electron microscope were used for evaluation of apoptosis. The expression of survivin was detected by RT-PCR and Western blot. Results ①Celecoxib inhibited cell survives in a time-dependent and concentration-depended manner, and the effect of celecoxib was more significant in NCI-H520 than in A549. ②Celecoxib caused apoptosis in concentration-depended manner. 3. Celecoxib decreased the expression of survivin. Conclusion The decrease of the expression of survivin may play a critical role in the apoptosis of NSLLC in vitro induced by celecoxib.
出处
《基础医学与临床》
CSCD
北大核心
2006年第8期856-859,共4页
Basic and Clinical Medicine
