摘要
采用单种卵囊分离技术建立了鹅艾美耳球虫（Eimeria anseris）克隆株，并对其基本生物学特性进行了研究。卵囊呈梨形，一端截平，另一端钝圆。卵囊壁单层，光滑无色或淡黄色，狭窄截平端有卵膜孔。无极粒，外残余体似一块不规则的塞状物，位于卵膜孔的正下方。测量100个卵囊，平均大小为19．37μm×16．06μm，形状指数为1．20。孢子囊卵圆形，斯氏体明显，有内残余体。测量100个孢子囊，平均大小为9．09μm×6．83μm。潜在期为168～173h，显露期为107～118h，最短孢子发育时间为55h。用不同剂量感染时，Eanseris的繁殖力差异显著（P〈0．05）。
A clone strain of Eimeria anseris was developed. The oocysts were shaped like a sphere surmounted by a truncate cone with a smooth, colorless, I-layered wall, a residuum and a micropyle ; without a polar granule. Oocysts were measured in 19. 37 μm×16.06μm, and the shape index was 1.20. The sporocysts were ovoid with a stieda body, and measured in 9. 09 μm×6.83μm. The minimum sporulatic time was 55 h; the prepatent period was 168 - 173 h; the patent period was 107 - 118 h. The reproductivity was affected by doses of oocysts significantly （ P 〈 0. 05 ）.
出处
《畜牧与兽医》
北大核心
2006年第3期20-22,共3页
Animal Husbandry & Veterinary Medicine
关键词
鹅艾美耳球虫
克隆株
建立
生物学特性
Eimeria anseris
clone strain
development
biological characterizations
作者简介
王留（1978-），男，硕士研究生。
通讯作者。
