摘要
目的:探讨钒配合物LMC对拓扑异构酶Ⅰ、Ⅱ(Topo-Ⅰ、Topo-Ⅱ)的影响及其抗肿瘤活性。方法:采用DNA松弛实验观察LMC对Topo-Ⅰ、活性的影响并探讨其相关分子作用机制;采用MTT法、流式细胞术在细胞水平观察了LMC的抗肿瘤作用。结果:LMC可明显抑制Topo-Ⅰ活性,对Topo-Ⅱ无明显抑制作用,对多种肿瘤细胞株A549、Hela、BEL-7402具有明显抑制生长的作用,且可将细胞阻断在G2/M期,而对正常细胞株L-02生长无明显影响。结论:钒配合物LMC具有抑制Topo-Ⅰ活性而发挥抗肿瘤的作用。
In this paper, we studied the effects of vanadium compound LMC on DNA topoisomerase and its antitumor activity. DNA relaxation assay was used to detect the activity of LMC on topoisomerase Ⅰ （Topo - Ⅰ ） and topoisomerase Ⅱ （Wopo - Ⅱ ） and its correlated molecular mechanism of action; MTr assay and Flow cytometry were used to detect LMC＇ s antitumor activity from cytology angle. LMC can inhibit Topo- Ⅰ activity obviously, but not, Topo - Ⅱ LMC can also inhibit A549,Hela,BEL- 7402 cell lines growth evidently, and LMC can arrest the ceils at G2/M stage. However, it makes no difference to normal cell llne L- 02, from which we conclude that Vanadium compound LMC has obvious anticancer activity through inhibiting Topo- Ⅰ activity.
出处
《现代生物医学进展》
CAS
2006年第4期13-15,共3页
Progress in Modern Biomedicine
基金
国家重点基础研究发展规划(973计划)资助项目(No.2003CB716400)
作者简介
莫晓媚，女，硕士，研究方向：分子药理学，Tel：0532—82031981。Email：silvia_mpl@163．com.
通讯作者：耿美玉（1963-），女，教授，博士生导师，研究方向：分子药理学。Tel：0532—82032066，Fax：0532—82033054。Email：gengmy@mial．ouc．edu．cn．
