摘要
目的探讨降钙素基因相关肽((CGRP)及其拮抗剂(h-CGRP 8-37)与正常和应激情况下大鼠胃内胆汁返流的关系。方法 SD大鼠85只,实验分三部分:第一部分大鼠20只随机分为四组:对照组和CGRP低、中、高剂量组各5只,分别腹腔注射生理盐水(1 ml)和CGRP(10 μg/kg 1ml)、CGRP(30μg/kg 1 ml)和CGRP(1 ml),0．5 h后处死,取胃液测胆汁酸(TBA)浓度。第二部分采用冷束缚应激法,将35只大鼠分为两组,对照组5只,实验组30只,从浸入水中开始取2、4、5、6、8、10 h共6个时段,每个时段各5只,分别检测其胃内胆汁酸的浓度和胃黏膜的溃疡指数(用Guth评分),用放免试剂盒检测幽门区CGRP含量。第三部分大鼠30只随机分为两组:应激组和h-CGRP 8-37组各15只,从浸入水中开始取2、4、6 h共3个时段,每个时段各5只。取胃液测TBA浓度。结果正常大鼠在腹腔注射不同剂量CGRP 0．5 h后胃内胆汁酸浓度明显增高;应激性溃疡大鼠于应激结束后2h(即从应激开始计时6 h)胃内胆汁酸达到峰值,溃疡指数和幽门区CGRP含量分别于应激结束后4 h达到峰值和降到波谷;经幽门局部给予CGRP拮抗剂能显著降低应激性溃疡大鼠胃内胆汁返流程度。结论 CGRP能促进胃内肌汁酸反流,CGRP参与了幽门括约肌舒张功能的调控。
Objective To study the effects of caleitonin gene-related peptide（CGRP） and its antagonist of CGRP（ h-CGRP 8-37 ） in normat and stress rats on bile reflux in stomach. Methods The study contained 85 SD rats and three parts. Part one：20 rats were randomly divided into control group and 10μg/kg, 30μg/kg, 50μg,/kg CGRP group. Peritoneal injection 30 rain later,the bile acid in gastric juice was measured. Part two：The stress ulcer model was established by cold,water seaking. 35 rats were divided into 0 h （control group） 2 h ,4 h ,6 h,8 h, 10 h stress group and measured the gastric ulcer index and bile acid in gastric juice. The pyloric local CGRP was measured by a radioimmunoassay detection kit. Part three：30 rats were divided into stress group and h-CGRP 8-37 group. The gastric bile acid were measured after 2 h,4 h,6 h stress. Each hour group have 5 rats. Results The bile acid in CGRP group increased significantly compared with those of control group. The bile acid reached the maximum at 2 hours after finish stress. The ulcer index reached the maximum at 4 hours after finish stress. But the pyloric local CGRP decreased to the minimum at 4 hours after finish stress, And,the bile acid of h-CGRP 8-37 group was less than those of antagonist control group. Conclusion CGRP can promote bile add reflux in the stomach. CGRP affect and regulate the relaxation function of the pyloric sphincter.
出处
《临床消化病杂志》
2006年第1期35-37,共3页
Chinese Journal of Clinical Gastroenterology
