摘要
目的以几丁质为载体,戊二醛为交联剂固定化粪产碱杆菌青霉素G酰化酶。方法单因素优化固定化条件,以交联度、pH、温度、酶量4因素3水平正交试验,确定最佳固定化条件,并研究固定化酶的最适反应温度、pH及批次稳定性。结果最佳固定化条件为几丁质0.3 g,酶量6.0 mL,交联度1.5%,温度37℃,pH 8.0,时间48 h,固定化酶最高比活性为62.3 U/g湿载体,最适反应温度为65℃,最适pH 9.0,重复使用8批活性基本稳定。结论戊二醛交联几丁质固定化青霉素G酰化酶稳定性较好,具有一定的工业应用前景。
Objective To immobilize penicillin G acylase from Alcaligenes faecalis with a crosslinked mixture of glutaraldehyde and chitin.Methods By the optimization of the immobilized conditions with the method of single factor analysis,the optimum immobilized conditions were determined by an orthogonal experiment with four factors(degree of cross-linking,pH,temperature,enzyme amount) and three levels.Finally,the optimum reaction temperature,pH and batch stability of immobilized enzyme were studied.Results The optimu...
出处
《食品与药品》
CAS
2010年第11期392-396,共5页
Food and Drug
基金
鲁东大学科研基金项目(LY20083305)
关键词
青霉素G酰化酶
几丁质
戊二醛
固定化
penicillin G acylase
chitin
glutaraldehyde
immobilization
