摘要
用镀铂玻碳电极电化学聚合３，３′－二氨基联苯二胺固定葡萄糖氧化酶（ＧＯＤ）制成电流型葡萄糖传感器．镀铂电极铂黑沉积层的微孔结构使电极具有较大的有效表面积，ＧＯＤ固定于铂黑微粒孔隙中具有较高的稳定性．传感器具有响应快、灵敏度高、线性范围宽、稳定性好等优点，并具有抗抗坏血酸、抗尿酸干扰的特点．
An amperometric glucose biosensor has been developed based on immobilization of GOD in electrochemically synthesized poly (3, 3′-diaminobenzidine) (PDAB) film on a platinized glassy carbon electrode(PGCE).The PGCE provides a large apparent surface area ,and GOD entrapped in the of the electrodeposited platinum particles is more stable than on a platinum disc electrode surface. The sensor respeonses to β-D-glucose linearly over a concentration range of 1×10(-6)to 1×10(-3)mol/L, and the detection limit is 5. 1×10(-7)mol/L.At a concentration of 0. 1 mmol/L glucose, the response current of the sensor is fifty fold higher than that of the sensor prepared with a platinum disc electrode.The response time of the sensor is less than 4 s and the life time is over 6 months.Due to the permselective characteristics of the PDAB film, the effects of electroactive interferents such as ascorbic acid and uric acid, can be significantly reduced.
出处
《高等学校化学学报》
SCIE
EI
CAS
CSCD
北大核心
1995年第12期1856-1860,共5页
Chemical Journal of Chinese Universities
基金
国家自然科学基金
中国科学院长春应用化学研究所电分析化学开放实验室基金
关键词
生物传感器
二氨基联本二胺
葡萄糖氧化酶
Biosensor,3,3′-Diaminobenzidine,Electropolymerization, Glucose oxidase.Platinized glassy carbon electrode
