摘要
目的 探讨转化生长因子 β(TGF_β)对面突外胚间充质干细胞向平滑肌细胞分化的影响。 方法 6 0pmol LTGF_β作用于面突外胚间充质干细胞 ,分别于 1d、2d后进行抗α平滑肌肌动蛋白 (α_SMA)单克隆抗体免疫组化图象分析检测 ,2d后定量RT_PCR检测α_SMAmRNA的表达。结果 免疫组化显示 ,TGF_β诱导组表达α_SMA的细胞数约为 95 % ,而未加分化抑制剂的自然分化组表达α_SMA的细胞数约为 6 5 %。图象分析显示 ,1d、2dTGF_β诱导组抗α_SMA着色平均灰度值小于未加分化抑制剂的自然分化组。定量RT_PCR显示TGF_β诱导组α_SMAmRNA量大于自然分化组。有分化抑制剂的对照组细胞不表达α_SMA。结论 TGF_β诱导组细胞表达α_SMA强于自然分化组。TGF_β可以促进外胚间充质干细胞向平滑肌细胞分化。
Objective To investigate the effect of transforming growth factor β(TGF-β)on ectomesenchymal stem cells differentiating to smooth muscle cells. Methods 60 pmol/L TGF-β was added to the ectomesenchymal stem cells of embryonic facial processes. Immunohistochemistry assay and image analysis were used to value the expression extent of α smooth muscle actin (α-SMA) and quantitative RT-PCR was used to value the quantity of α-SMA.Results 2 days later, about 95% cells in TGF-β group and 65% cells in control group without differentiation inhibitor expressed α-SMA. Expression of α-SMA in TGF-β group was stronger than that of control group after one and two days. Quantitative RT-PCR showed the quantity of α-SMA mRNA in treated group cells was more than that of in control group. Conclusion Quantity of α-SMA in TGF-β group is more than that of spontaneous differentiation group. TGF-β has positive effect on ectomesenchymal stem cells differentiating to smooth muscle cells.
出处
《华西口腔医学杂志》
CAS
CSCD
北大核心
2004年第6期460-462,共3页
West China Journal of Stomatology
基金
第四军医大学创新工程重点课题资助项目 (CX 0 2F0 0 2 )
关键词
外胚间充质干细胞
转化生长因子
平滑肌细胞
分化
ectomesenchymal stem cell
transforming growth factor β
smooth muscle cell
differentiation
